M1L2 - Peptide purification

Dialysis

This separates molecules based on size via a semipermeable membrane with small pores

Gel filtration

This separates molecules based on size, with smaller molecules passing through a matrix of gel beads

Electrophoresis

This separates molecules based on net charge at a chosen pH

Ion exchange

This separates molecules based on net charge using an ion exchange resin

Cation exchange

This is an ion exchange that binds cations and is itself negatively charged

Anion exchange

This is an ion exchange that binds anions and is itself negatively charged

Diastereoisomer formation and hydrolysis

This reacts the enantiomeric mixture with another pure enantiomer to form diastereoisomers which can then be separated

Chiral counterion crystallisation

This reacts an enantiomeric mixture with a chiral salt, then purified and separated

Enzymatic resolution

This separates enantiomers based on their interaction with enzymes e.g. acylase

Chiral chromatography

This is a type of chromatography with a chiral solid phase, allowing for the resolution of a racemic mixture