DFS, Iodine Staining, KOH Wet Mount (Part 1)

● Stool

○ Most commonly submitted sample for the

examination of parasites because the

largest number of parasites affecting

man affects the intestines.

○ Very important in detecting protozoa.

○ In (_____) protozoa, we can find here cysts

(stage ?)/trophozoid stage, helminth

eggs, larva, adult worm/proglottids in

cestodes.

● Tissue aspirate

○ Not a common specimen.

○ For tissue aspirate, we can use liver

aspirate, duodenal aspirate or

bronchoalveolar lavage.

● Blood

○ For blood specimens, we can find

plasmodium species, Leishmania

species, or Trypanosoma species.

● Urine

○ We can use (________) samples in detecting

Trichomonas vaginalis and

Schistosoma haematobium.

● Sputum

○ Detects specifically, Paragonimus

westermani.

● Muscle Biopsy

○ Detects Trichinella spiralis.

● Cerebrospinal Fluid

○ This is the site where the

Acanthamoeba goes (in the nervous

system). Acanthamoeba is contracted

when swimming in contaminated pools

when you are immunocompromised.

● Orifice Swab

openings in the body e.g. vagina

(where we can detect Trichomonas

vaginalis), and perianal areas where we

could get Enterobius vermicularis and

Taenia species.

Routine Ova and Parasites (O&P).

Most common procedure performed in the

examination of stool specimen, also called as the

Routine Stool Examination. In some books, they

call it

collected every other day or

a total of 3 collected in 10 days.

Patient Preparation

The typical stool collection protocol consists of 3

specimens: one specimen

(3-4) (5-6)

A patient that has received treatment for

protozoan infection should be checked (__) weeks after

therapy and those treated with Taenia infection should be

checked (___) weeks after therapy.

Tight fitting lid

= to avoid spillage and to contain the

moisture within the container.

Wide mouth

= facilitate in the collection (makes the

collection easier).