DNA (structure, Function, Replication)

biol 1200 summer

What is the structure of DNA?

Double helices anti-parallel (5→ and 3←5) held by hydrogen bonds between bases w/ sugar phosphate backbone

What is a nucleotide consisted of?

Nucleotide= Deoxyribose sugar, phosphate group(s), and nitrogenous base (A,C,G, T)

Prokaryotes vs. Eukaryotes (chromosone organization)

Prokaryotes are single celled that lack a nucleus, while eukaryotes have a nucleus and can be both

What region does Polymerase Chain Reaction (PCR) copy?

Specifically copies ONE region of DNA

How many copies does PCR make?

Typically millions of copies

What is PCR created with?

Uses primers, heat and DNA polymerase.

What are the DNA isolation steps?

1) cell lysis to break down the cellular membrane, 2) precipitation of DNA using alcohol or salt, 3) purification to remove proteins and other contaminants.