Bioluminescence metabolism overview

These will be a mixture of the fungal and bacterial bioluminescence metabolic pathways

R-CHO

Long-chain aldehyde

What enzyme is responsible for reducing FMN to FMNH2? Is this a primary or secondary pathway?

NAD(P)H-FMN oxidoreductase. Secondary.

R-CHO

Long chain aldehyde is synthesized from fatty acid precursors through the fatty acid biosynthesis pathway

Armillaria mellea (honey fungus)

An edible basidiomycete

Carbon supply in fungal bioluminescence pathway

Malonyl CoA and Caffeic Acid

Precursor to Malonyl CoA

Acetyl CoA

Malonyl CoA

central metabolite in fatty acid biochemistry

Cofactors in the fungal bioluminescence pathway

ATP, NAD(P)H, 4’phosphopantetheine, O2

NADPH, in the fungal bioluminescence pathway, is produced via what pathway?

Pentose phosphate pathway

Hispidin Synthase

converts caffeic acid to Hispidin

What role does Hispidin-3-Hydroxylase (h3h) play in fungal bioluminescence?

H3h hydroxylates Hispidin into 3-hydroxyhispidin, or, Luciferin

Oxidizes luciferin to produce a high energy, unstable, intermediate that emits light as it becomes oxyluciferin

The role of luciferase in the fungal bioluminescence pathway

Oxyluciferin can be recycled into caffeic acid by?

caffeylpyruvate hydrolase (cph)