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why are inks used to dye tissue?
To mark important orientation, anatomic landmarks, lesions, and margins
How can ink be adhered better to the tissue?
Dry the surfaces of the specimen before applying ink
Inking procedure:
Apply ink as designated in your gross
Repeatedly dab excess ink from the specimen
(dry again?)
use vinegar as a mordant, allow the vinegar to stand on the ink for 10-15 secs
Allow ink to dry before maing any cuts
Why shouldn’t you cut into the specimen before the ink is dry?
If the ink is not dry, could result in dragging ink to an area it shouldn’t be (false margin)
what must be included in an official gross photograph?
Associated gross evaluation and description
Why would we want to use gross photography?
document interesting gross findings
support publications for research or case studies
Guidelines for gross photography:
Clean background (no blood, fluid, tools)
Centered
Ruler for scale
Patient identifiers/surgical path number
take one picture showing the specimen overall
take a macro photo
Save the photo
What are two options of gross photography equipment?
iphone/digital camera
photograph station, bench
What is the standard rule of sampling a tumor?
1 section per 1 cm, meaning for every 1 cm of tumor, the PA would submit 1 section
If you had a 16 by 14 by 10 cm mass, how many sections would you need to submit?
16 sections of the mass
What are some examples of specimens that would be used for tumor mapping?
Osteosarcoma
Prostate
Breast
vulvectomy