Hematology & Coagulation Reference Ranges/Testing

RBC reference range? (Both female and male) (2)

• Female: 4.2-5.4 ×10^12/L

• Male: 4.7-6.2 ×10^12

Normal blood pH?

7.35-7.45

Normal WBC count?

4.8-10.8 ×10^9/L

Normal platelet count?

150-400 ×10^9/L

Normal Percent of segmented neutrophils? bands?

• 50-70%

• 2-6%

Normal percent of lymphocytes?

20-44%

Normal percent of monocytes?

2-9%

Normal percent of eosinophils? Basophils?

• 0-4%

• 0-2%

Normal M:E ratio in the bone marrow?

2:4 (LOOK UP)

Normal reticulocyte count?

0.5-2.0%

Normal hemoglobin amount (Men and women) (2)

• Men: 14-18 g/dL

• Women: 12-16 g/dL

Normal hematocrit amount? (men and women)

• Men: 42-52%

• Women: 37-47%

Reference range for MCV? Calculation?

• 80-100 fL

• (Hct x 10)/RBC

Reference range for MCH? Calculation?

• 27-32 pg

• (Hgb x 10)/RBC

Reference range for MCHC? Calculation?

• 32-36%

• (Hgb x 100)/Hct

RDW reference range?

11.5-14.5

Calculation for hemocytometer (body fluids)?

(# of cells x dilution factor)/(area counted x depth)

WBC and platelet estimates?

• WBC: average number of WBC counted in 10 fields x2,000

• Platelets: average number counted in 10 fields x 15,000

Retic count calculations (with and without miller disc)

• With miller disk: [(total retics counted)/(total RBCs x9)] x100

• Without miller disk: (# of retics counted out of 1000 RBCs)/10

Absolute reticulocyte count

(reticulocyte(%) x RBC count)/100

Corrected reticulocyte count?

(% reticulocyte count x patient Hct)/45 (avg. hct)

Keilhauer betke stain calculations:

• % of cells containing fetal hemoglobin

• Volume of fetal maternal hemorrhage

• Number of RhoGAM vials

• (# of fetal cells)/2000

• (% cells containing hgb F) x 50

• (volume of fetal maternal hemorrhage)/30