Biotechnology and microscopy

Which microscope use visible light to view a thin sample?

light microscope

Which microscope is used to view surface of 3D object with high resolution?

scanning electron microscope

Which microscope is used to view thin cross sections and internal structures within samples at very high magnification?

Transmission Electron Microscope

Which microscope can observe specific parts of a cell using fluorescent tagging antibodies?

Confocal Laser Scanning Microscope/Fluorescence

Which layer form a pellet at bottom of sample in centrifugation?

Most dense

What is centrifugation?

A technique to separate a liquified sample into its different components by spinning it rapidly

What is Gel Electrophoresis?

A technique to separate DNA molecules by size and charge

Do small molecules travel farther or closer down the gel?

Farther

What is microarray assay?

A technique to monitor the expression of large groups of genes across a genome

What is DNA sequencing?

A technique used to determine the sequence of base pairs in a DNA or RNA molecule

What are blotting techniques?

A technique to identify specific fragments of DNA, RNA, or protein

What is the function of restriction enzyme?

To cut specific segments of DNA called restriction sites

What is the function of reverse transcriptase?

Used to synthesize DNA from a DNA template

What is Polymerase Chain Reaction (PCR)?

A technique to creates a large amount of DNA by amplifying a DNA sample