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Accident or injury reporting
If an accident or injury occurs in the lab, you should report it to the teacher.
Fire safety tools
In the event of a fire, a fire blanket or fire extinguisher can be used.
Test tube safety
You should never stopper a test tube while heating it because it can explode.
Most important safety rule
Always follow directions.
Organism
An organism is the biological term for a living thing.
Line graphs
Line graphs usually show how one or more variables changes over a period of time.
Measurement units
Meters measure distance, liters measure volume, & Mass is measured in grams.
Centrifuge
A centrifuge separates material according to density.
Gel electrophoresis
Gel electrophoresis uses electricity to separate DNA fragments.
Indicator
An indicator changes color in the presence of a specific substance.
Compound light microscope
This microscope we use in class utilizing two lenses is called a compound light microscope.
Microscope image orientation
Image always appears upside down and backwards.
Ocular or eyepiece
Piece of microscope you look through usually magnifies 10 x.
Total magnification
Total magnification is found by multiplying the magnification of the ocular x objective.
Field of view
The area you can see when looking through a microscope is called the field of view.
Field of view under high power
Under high power, the field of view is smaller.
Image brightness under scanning power
An image is brighter under scanning power.
Diaphragm function
The diaphragm controls the brightness, amount of light.
Wet mount slide preparation
When preparing a wet mount slide, always place the slide cover at an angle to minimize air bubbles.
Staining a specimen
Staining a specimen is used to make cell parts more visible; staining kills the specimen.
Electron Microscopes
Electron Microscopes have the highest magnification; they can see viruses.
Scientific Inquiry
Scientific Inquiry is the development of explanations and theories.
Observation
An observation is information collected using the five senses.
Inference
An inference is not always a fact; it is a conclusion based on observations.
Scientific method
The scientific method is a process of organizing experimentation to solve a problem.
Steps of the scientific method
The 7 steps of the scientific method are define the problem, gather data (research), make a hypothesis, perform a controlled experiment, collect & analyze data, draw a conclusion, repeat.
Problem statement
A problem must be written in the form of a question.
Hypothesis statement
A hypothesis must be written in the form of a statement.
Controlled experiment groups
In a controlled experiment, you should have 2 groups: controlled group and experimental group.
Independent variable
All conditions will be kept the same between the two groups EXCEPT for the one factor being manipulated, this is the variable I control, known as the independent variable (the cause).
Dependent variable
The dependent variable is what is being affected by the independent variable.
Hypothesis 'if' portion
In a hypothesis, the 'if' portion of the statement is the independent variable.
Control group
The standard to which comparisons are made in an experiment is called the control group.
Constant variable
A constant variable is any variable that is the same for all test subjects.
Dependent variable graphing
When graphing your results (data), the dependent variable goes on the Y-axis.
Conclusion
Your conclusion must either support or refute your hypothesis.
Good experiment practices
Three ways to ensure a good experiment are use a large sample size, have only 1 independent variable, repeat the experiment.
Theory
An explanation of a natural phenomenon supported by many observations (evidence) and experiments over time is a theory.
Ethics
Ethics are moral values or principles.
Energy storage
Energy is stored in chemical bonds.
Inorganic compounds
Inorganic compounds can include any element but cannot contain both Carbon & Hydrogen.
Organic compounds
Organic compounds must contain Carbon AND Hydrogen.
Inorganic molecules examples
H2O, CO2, and O2 are examples of inorganic molecules.
Organic compounds list
The 4 organic compounds are carbohydrates, lipids, nucleic acids, proteins.
Lipid subunit
The subunit of a lipid is made of 1 glycerol and 3 fatty acids.
Starch composition
A starch is made up of simple sugars (monosaccharides - GLUCOSE).
Carbohydrates vs Lipids
Carbohydrates serve as an energy source, while lipids store energy.
Carbohydrates structure
Carbohydrates have ring-shaped molecules.
Elements in carbohydrates and lipids
Carbohydrates and lipids contain the elements carbon, hydrogen, and oxygen, but only carbohydrates have a 2:1 ratio of Hydrogen to Oxygen.
Nucleic acids building blocks
The building blocks of nucleic acids are nucleotides.
Nucleotide composition
A nucleotide contains a phosphate, sugar, and nitrogen base.
Proteins building blocks
The building blocks of proteins are amino acids.
Elements in proteins
Proteins contain the elements carbon, hydrogen, oxygen, nitrogen.
Protein function
A protein's shape defines its function.
Enzymes
Enzymes are a type of protein.
Chemical reaction results
In chemical reactions, the end results are the products.
Synthesize
To synthesize means to build or make a larger molecule from smaller ones.
Digest
To digest is to break down (lysing) molecules into smaller ones.
Catalyst
A catalyst speeds up a reaction.
Enzymes in reactions
Enzymes help speed up a reaction but are not used in the reaction so can be reused.
Lock and key model
The lock and key model states each enzyme's specific shape only fits with one substrate.
Factors affecting enzymes
The factors that affect enzymes are temperature, pH, and enzyme-substrate concentration.
pH scale
The pH scale measures acids and bases.
pH
The more acidic, the lower the pH.
Benedict's solution
Tests for glucose.
Iodine
Tests for starch.
Photosynthesis
Taking CO2, water, and sunlight to make glucose and oxygen.
Cellular respiration
The opposite of photosynthesis.
Metabolism
The sum total of all life activities.
Regulation
The control and coordination of activities of organisms. Response to stimuli.
Synthesis
To build. Using smaller molecules to make larger ones.
Transport
The absorption and circulation of materials throughout an organism.
Respiration
The life process involved in breaking down glucose to generate energy.
Nutrition
Involves three processes: ingestion, digestion, egestion.
Autotrophs
Can make their own food (plants, some bacteria).
Heterotrophs
Organisms that cannot make their own food.
Growth
The increase in cell size and/or the number of cells in an organism.
Excretion
The removal of the waste products produced by the organism (metabolic wastes).
Reproduction
Not needed by the individual to survive, but is needed by the species.
Homeostasis
Depends on all life functions working together. Keeps the organism stable (healthy).
Levels of organization
From small to large: molecule, organelle, cell, tissue, organ, organ system, organism.
Prokaryotic cells
Do not have a nucleus or membrane-bound organelles.
Eukaryotic cells
Have a nucleus that contains DNA.
Cell membrane
The barrier around the cell that regulates what enters and exits.
Nucleus
The control center of the cell.
Ribosomes
The site of protein synthesis.
Cytoplasm
Fluid within the cell, primarily made up of water.
Mitochondria
The site of cellular respiration (breaking down glucose to release energy in the form of ATP).
ATP
Adenosine triphosphate... is energy.
Chloroplasts
Where photosynthesis occurs in plants.
Vacuole
Stores food, water, and waste in a cell.
Selectively permeable membrane
Allows some substances in and keeps others out.
Phospholipid bilayer
The cell membrane is primarily made of a phospholipid bilayer (2 layers of lipids) and proteins.
Fluid-mosaic model
Describes how the components of the cell membrane are not in fixed positions.
Diffusion
Requires no energy. Movement from high to low concentration.
Active transport
Requires energy to move materials across the cell membrane from low to high concentration.
Osmosis
Diffusion of water through a selectively permeable membrane.
Hypertonic solution
When placed in a hypertonic (salty) solution, an animal cell will shrivel and a plant cell vacuole will collapse.
Hypotonic solution
Has a lower concentration of solute than inside the cell, so water will diffuse into the cell.
Starch
A large molecule that will not pass through a cell membrane.