pregnancy test MOA
Detect presence of human chorionic gonadotropin (hCG) by using monoclonal or polyclonal antibodies in an enzyme immunoassay
what are the two types of preg tests
qualitative
quantitative
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pregnancy test MOA
Detect presence of human chorionic gonadotropin (hCG) by using monoclonal or polyclonal antibodies in an enzyme immunoassay
what are the two types of preg tests
qualitative
quantitative
when should you pee on a preg test
first pee of the day
why is first pee of the morning best for preg tests?
concentration
when should you test the urine sample for a preg test
asap
if you cant test a urine sample for a preg test immediately, what should you do?
refrigerate it and then take it out to warm to room temp for 20-30 mins before testing
it may take at least ______ post ovulation for egg to implant and begin to have detectable levels of hCG
5-11 days
it may take at least 5-11 days post ovulation for egg to implant and begin to have detectable levels of ___
hCG
it is best to wait until ___ to test for preg
day period is due or the day after
(14-15 days after ovulation)
what can interfere with preg test results
Miscarriage or birth within the previous 8 weeks
Medications used for fertility (gonadotropins)
what are the options to predict ovulation
Basal body temperature (BBT) thermometers
Ovulation prediction kits (OPKs)
Ovulation detection devices
MOA of basal thermometry testing for ovulation
basal body temp to predict
a ____ in temp signals that ovulation has occured
increase
normal BBT in women
97-97.5 degrees
BBT in women 24-48 hours after ovulation
98.6
basal thermometry ovulation test requires
basal thermometer duh
what can interfere with the results of basal thermometry testing
Emotions, movements, and infections can influence the BBT
Eating, drinking, talking, and smoking within 30 minutes of checking one’s temperature should be postponed until after a measurement is obtained
MOA of urinary hormone ovulation tests
Use monoclonal antibodies specific to luteinizing hormone (LH) in urine samples to detect the surge in LH prior to ovulation (some also test for estrogen)
generally urinary hormone ovulation tests recommend to use
first morning pee
when should urinary hormone ovulation testing be done
starting on the day of the menstrual cycle recommended by the product, until results indicate high or peak fertility
what can cause false positive results in a urinary hormone ovulation test
Medications used to promote ovulation
Menopause and polycystic ovarian syndrome (PCOS)
Pregnancy
what can interfere with urinary hormone ovulation testing
If previously on oral contraceptives, avoid use until 1 to 2 months after discontinuation
low sperm count
<15 million/ml
moderate sperm count
15-55 million/ml
optimal sperm count
>55 million/ml
MOA of male fertility testing
measures sperm count
assess sperm volume, motility, and morphology
in most cases ___ tests are needed to validate mens fertility status
2
fecal occult blood tests MOA
Detect blood in feces with a colorimetric assay for hemoglobin (Hg), the heme portion of Hg acts as an oxidizing agent to catalyze oxidation of the test reagent, tetramethylbenzidine, producing a blue-green color
what is the testing reagent for the fecal occult blood test
tetramethylbenzidine
which color indicates a positive fecal occult blood test
blue/green
what are the two types of fecal occult blood tests
toilet test
stool application products
who is excluded from self testing FOBT
ppl on they periods (gotta wait till its over)
what could interfere with the results of a FOBT
Blood in the stool from other conditions
Use of aspirin, NSAIDs and steroids
Rectally administered medications
Some medications
NSAIDs, Vitamin C, iron
Some foods
Toilet bowl cleaner
when testing for fecal occult blood, you should avoid the use of aspirin, NSAIDs and steroids for…
2-3 days before testing and during the testing period
when testing for fecal occult blood, you should avoid the use of _____ for 2-3 days before testing and during the testing period
aspirin, NSAIDs and steroids
which medications can interfere with FOBT
NSAIDs, vitamin C, iron
what could be measured in a cholesterol test
total cholesterol (TC), LDL-C, HDL-C, and triglycerides (TG)
who is excluded from self testing cholesterol
coagulation disorders or anticoagulant use
cholesterol testing requires
blood sample
what can interfere with cholesterol testing
Excessive squeezing and milking of the finger
Blood sample too small or prolonged collection (> 5 minutes)
High doses of Vitamin C (≥ 500 mg)
uses of UTI testing
For early detection of UTI in patients with a history of recurrent UTIs or risk factors associated with UTIs
Confirmation that an infection has been cured by antibiotic therapy
MOA of UTI test
Detection of both nitrite and leukocyte esterase (LE)
UTI testing requires a bacterial conc of ___ in the urine
10^5 per ml of urine
gram - bacteria reduce nitrate to ___ in the urine
nitrite
Arsanilic acid reacts with urinary nitrite to form a diazonium compound, which reacts with another chemical on the test to produce a ___ color
pink
leukocyte esterase in UTI testing only helps to identify…
gram + organisms
what could cause a false negative UTI test
Strict vegetarian diet
Tetracycline use
Vitamin C doses greater than 250 – 500 mg
what could cause a false positive UTI test
◦ Dyes or medications such as phenazopyridine
what kinda pee do u want for a UTI test
first morning pee
held bladder for at least 4 hours pee
HIV test MOA
Use blood or oral fluid samples to detect antibodies to the virus (Sufficient antibodies must develop for detection)
what could interfere with HIV testing
inadequate blood sample or inadequately swabbing the gums
pts taking immunosuppressant therapy
Patients taking immunosuppressant therapy may not be able to produce sufficient levels of ABs adn may obtain a ____ result for a HIV test
false negative
what should not happen 30 mins before an HIV test
eating
drinking
use oral care products
MOA of HepC tests
Tests for presence of antibodies to the hepatitis C virus, not the virus itself
Uses an enzyme immunoassay to test for antibodies in blood sample
____ may be required for enough ABs to detect hepC
6 months
you have to send the home blood sample for which test to a lab to process
hepC
what could interfere with Hep C test
inadequate blood sample
immunosuppressant therapy
immunosuppressant therapy could provide a ___ test result for hep C
false negative
drug testing requires
urine or hair
u gotta mail the sample to a clinical lab for
drug testing
what could cause a false positive drug test
decongestants, dextromethorphan, antidiarrheals, or cough medicines containing codeine, consumption of large amounts of poppy seeds
urine drug tests are better for detecting
low-level, casual drug use
hair drug testing is better for detecting
long term use
how big should the hair be for drug testing
½-inch wide and 1 ½ inches in length from the crown of the head, as close to the scalp as possible