Base
Bottom of the microscope, where to support with palm
Arm
Grip for microscope when carrying
Platform
Support specimen being observed
Diaphragm
Controls light that goes through specimen
Stage Clips
Holds down specimen
Mirror
Light source
Low power objective lens
4x magnification
Medium power objective lens
10x magnification
High power objective lens
40x magnification
Body tube
Creates magnified image using light from objective to pass upward
Ocular
Further magnifier by 10x
Coarse adjustment knob
Moves stage up and down
Fine adjustment knob
Minute focusing on specimen
Rotating nosepiece
Objective switcher
Why is the image in the microscope flipped?
This is because there are two lenses in the microscope.
When can you use the coarse adjustment knob?
Only on low power
What would be the magnification of low power after multiplying by the 10x from the ocular?
40x
What would be the magnification of medium power be after multiplying by the 10x from the ocular?
100x
What would be the magnification of high power be after multiplying by the 10x from the ocular?
400x
Which is smaller, the coarse or fine adjustment knob?
The fine knob
Why can the coarse adjustment knob only be used on low power?
It can crack the slide.
Why can direct sunlight not be used to observe a specimen?
It can damage the eyes.
What is the most familiar type of microscope?
The compound light microscope.
Nucleus
Structure which contains DNA and controls all cell functions
Nucleolus
Prominent structure in nucleus that makes ribosomes
Nuclear membrane
The part of the cell that envelops the nuclear material and controls what enters and leaves the nucleus
Cell membrane
Semi-permeable structure that separates one cell from the next and controls what enters and leaves the cell
Cytoplasm
Jelly-like substance that is the fluid portion of the cell that helps a cell keep its shape
Golgi body
Responsible for storage and secretion of cell products and modifies, sorts, and packages and then stores materials and proteins from the rough er
Ribosomes
The cellular site of protein synthesis that produces proteins and is in the cytoplasm
Centrioles
Plays a key role in cell division
Rough endoplasmic reticulum
Structure that acts as a transport tube for cell proteins and directs them to where they are supposed to go; has ribosomes on the surface
Smooth endoplasmic reticulum
Creates lipids for the cell and breaks down toxins; does not have ribosomes on the surface
Lysosome
Source of digestive enzymes that break down food into small molecules
Mitochondria
Site of cellular respiration, where ATP energy for life activities is created; powerhouse of the cell
Chloroplast
Contains chlorophyll; The site of photosynthesis that captures sunlight for the plants to make into chemical energy(glucose)
Vesicle
Small sac for storage and transportation of materials through cell organelles that is made by the golgi apparatus
Vacuole
Large sac that gives the cell its shape and is filled with water, ions, and nutrients
Cell wall
Rigid layer(cellulose) that provides support and structure
Cytoskeleton
Organizes the cell and gives it formation and shape
Lipid Bilayer
Flexible double-layered sheet that makes up the cell membrane and forms a barrier between the cell and its surroundings
Which organelles are found only in plant cells?
Central Vacuole, chloroplast, and cell wall
Which organelles are not found in plant cells?
Centrioles and lysosomes
What is Trichology?
The scientific study of the structure, function, and diseases of human hair.
What is a wet mount?
Adding a drop of water to the specimen and holding the cover slip at a 45 degree angle before placing it on top the specimen
What are the 3 layers of a hair shaft?
Cuticle(the outer layer), the cortex, and the medulla
What is the cuticle?
The outer layer of the hair shaft that consists of overlapping scales that are directed toward the tip of the shaft.
What is the cortex?
The inside of the hair shaft that is made up of compact, elongated cells and often contains pigment granules.
What is the medulla?
The central core of the hair shaft that is composed mostly of air spaces. It can be either absent, continuous, or fragmented.
What does a cell membrane have the same consistency of at normal temp?
Olive oil
What is found on the surface of some proteins(glycogen)?
Carbohydrates which are important for cell-to-cell recognition
What is cholesterol to the cell membrane?
An integral component of the cell membrane which has a hydrophobic tail and a hydrophilic head which helps maintain integrity of the membrane which decreases fluidity, immobilizing outer surface. Helps secure important proteins to the membrane.
What does ATP stand for?
Adenosine Triphosphate
What happens when the bond between the last two phosphates is broken(hydrolyzed)?
A large burst of stored energy is released.
What is the uncharged form of ATP?
ADP
Where is all the energy stored?
In the last two phosphates
Where is movement during active transport?
Against the concentration gradient. This is when substances move from a low to high concentration
What are channel pumps?
Certain channel proteins in the cell membrane push molecules against their concentration gradient to maintain unequal levels using ATP. They work into or out of the cell.
What is the sodium-potassium pump?
Located on the cell membrane of all animal cells, responsible for 1/5 of total ATP, pumps sodium(Na) of out cells and potassium(K) into cells
Membrane potential
An electrical charge that exists across the cell membrane
Uniport
Moves ions/molecules in one direction
Antiport
Moves ions/molecules in opposite directions(against each other)
Symport
Moves two different ions in the same direction
What do vesicles have?
A phospholipid bilayer.
Endocytosis
The movement of substances into a cell by means of a vesicle.
What is pinocytosis(cell drinking)?
The process of taking up liquid from the surrounding environment(extracellular fluid). Tiny pockets form along the membrane, fill with liquid, and pinch off.
What is phagocytosis(cell eating)?
Extensions off cell membrane surround a particle and package it within a vesicle and then the cell engulfs it.
What is macrophages?
White blood cells
Exocytosis
The movement of substances out of a cell by means of vesicle which is done by the golgi apparatus to export proteins.
What are the methods of active transport?
Channel pumps and vesicles
What are the three rules of cell theory?
1: All living things are made up of cells.
2: Cells are the basic unit of structure and function in all living things.
3: All cells come from pre-existing living cells.
Prokaryotic
Simple in structure; no membrane bound organelles; unicellular; cell wall for protection and support which allows bacteria to live in varied environments
Eukaryotic
Complex in structure; membrane bound organelles;
Bacteria
Microscopic living organisms; among oldest living organisms; some are harmful some are good
What can prevent bacteria spread?
(Usually) antibiotics, antiseptics, disinfectants, and sterilization
Antibiotics
Selective poisons that seek to kill specific bacteria but they cannot kill viruses
Probiotics
“Good bacteria” that offer some type of health benefit to the host
How are bacteria antibiotic resistant?
They reproduce very quickly and therefore evolve quickly along with mutations, people not taking antibiotics for as long as they’re prescribed, and overuse of antibiotics.
Bacteria ways of movement:
Cilia and Flagella
Bacteria ways of obtaining energy
Photosynthesis, chemosynthesis, decomposers
Name three types of main bacteria:
Coccus, bacillus, and spirullus
Archaebacteria/extremophiles
Oldest known organisms; beginning of life on earth?- chemosynthesis; live in extreme environments
Eubacteria
Current form of bacteria; live all around us; constantly evolving
Name the extreme environments archaebacteria have been found in: ____philes
Thermophiles- Heat addict
Halophiles- Salt addict
Alkaliphiles- Base addict(pH 9+)
Acidophiles- Acid addict(pH 3-)
Psychrophiles(cryophiles)- Cold addict
Cellular transport
Movement of materials across the semi-permeable cell membrane to maintain homeostasis.
Hypertonic
More water in the cell than in the solution ex: cell-90% solution- 85% water
Hypotonic
More water in solution than in cell ex: cell-85% solution- 90% water
What are the two kingdoms or bacteria?
Archaebacteria and Eubacteria
Difference between homeostasis in unicellular and multicellular organisms
Unicellular organisms have one cell that performs homeostasis and performs all tasks while multicellular organisms have cells with specialized tasks that communicate to maintain homeostasis
What is cystic fibrosis?
When the cells that control mucus and sweat are disordered and then in turn leads to mucus build up that damages airways and the lungs.
Label a microscope
Passive Transport
Movement across the cell membrane that does not require any energy to occur; occurs due to concentration gradient; high concentration to low concentration
What are the three types of passive transport?
Diffusion, osmosis, and ion channels
Diffusion
Movement of a substance down the substance’s concentration gradient from high to low concentration; dissolved particles that are small and nonpolar can diffuse directly through cell membrane(Co2 and Oxygen)
Concentration gradient
A difference in the distribution of substances across a space
Equilibrium
A condition in which the concentration of a substance is equal throughout a space
Solute
Substance being dissolved
Solvent
Dissolver
Osmosis
Movement of water across a semi-permeable membrane through aquaporins
What happens when water moves out of the cell? What about in?
Out: Cell shrinks(hypertonic)
In: Cell enlarges(hypotonic)