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Description and Tags

histogram charts, electrophoresis, more junk for machines

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34 Terms

1
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normal RBC

2
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RBC agglutination

3
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Dimorphic RBC, RBC fragments, elevated RDW

4
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macrocytic RBC, elevated RDW, shift to the right

5
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microcytic RBC, elevated RDW, shift to the left

6
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normal WBC

7
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WBC shift to the left BLASTS

  • increase in immature granulocytes, abnormal cell populations, eosinophilia (R3 flag)

8
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immature granulocytes

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lymphocytosis

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variant lymph

11
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cellular interference

12
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eosinophilia

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blasts

14
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normal PLT

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small PLT

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giant PLT

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rbc and PLT overlap

18
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<p></p>

scatter plot

19
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how to fix cold agglutinins

warm specimen at 37 C for 15 min the re-run

20
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how to fix lipemic sample

  • perform Hgb determination on plasma supernatant, then subtract plasma Hgb value from original blood specimen hemoglobin

  • saline replacement - replace patients plasma with isotonic saline, remember to put same amount of saline as the amount of plasma taken out

21
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how to fix sample with leukocytosis

  • dilute specimen and multiply cell counts by DF

22
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<p>what does patient 1 have?</p>

what does patient 1 have?

Hgb S electrophoresis

23
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<p>what does patient 2 have?</p>

what does patient 2 have?

S trait (AS)

24
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<p>what does patient 3 have?</p>

what does patient 3 have?

Hgb C

25
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<p>what does patient 4 have?</p>

what does patient 4 have?

Hgb SC

26
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<p>what does patient b have</p>

what does patient b have

thalassemia barts, hydrops fetalis, a thalassemia major

27
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errors that happen when cold agglutinins are present

  • ↓ RBC

  • ↑ MCV, MCHC, HGB, MCH, grainy appearance

  • will show as dual population on rbc map or right shift on histogram

28
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what happens with a lipemic sample

  • ↑ HGB, MCH, MCHC

  • turbidity affects spectrophotometer in reading hgb

29
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what happens with a sample when it is hemolyzed

  • ↓ RBC

  • ↑ HCT

30
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what happens with a lysis resistant RBC (abnormal hgb) sample

  • ↑ WBC, HGB

  • HGB S, C, OR F may fail to lyse and will be counted as wbc

31
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what happens when a sample has microcytes or schistocytes

  • ↓ RBC

  • ↑ PLT

32
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what happens with a sample when there is NRBC

  • ↑ wbc

33
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what happens when there is platelet clumps

  • ↑ WBC

  • ↓ PLT

  • redraw with sodium citrate and multiply by 1.1

34
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what happens when WBC is increased (>100,000)

  • ↑ HGB, RBC, HCT, MCH, MCHC