glyconojugates

what is inhibited by tunicamycin

n linked glyc

what is the primary of glycoprotien and proteoglycan

for glycoprotein it is protein, for pro. it is carbohydrate and it is more neg. charged

how do fatty acid and sphingosine link

it is through amide linkage rather than ester linkage like lipids

what is glycosylation

enzyme mediated modification

Examples of N linked glyc

antibodies ig, glycoprotiens iib, iiia on platelets(clotting), gp120(hiv)

is o linked or n linked enzymes membrane bound

o linked

which one needs dolichol pp olignosaccharide

n linked

what are proteoglycan major components of

connective tissue , it has strong support and has signaling receptors for growth factors, cytokine

what is the glycoprotiein chains like

hort chain, branched, neutral, non repeating, olignosaccharideds

what is the proteoglycan chain like

long chain, linear, negatively charged, repeating disaccharide, glycosaminoglycans (gag)

how are glycosaminoglycans (gag) attached to a ser on the core

they bind covalently by xyl-gal-gal

is the backbone of sphingolpids glycerol

no it is sphingosine

what can ABO antigen attach to

both proteins and lipds on surface on red blood cells

what is glycation

monosaccharide (glucose) attaching nonenzymatically to amino group of protein

Which two glyco protines are in the inner membrane

o-glcnac glycoproteins, and cytoplasmic glycoprotiens

Function of glycoconjugates

cell-cell adhesion, cell signaling, receptors for virus/bacteria, gene regulation, immune responce, protein sorting

what activates sugars

UTP so they can bind to protein. this is specific for O linked glyco. for o you are attaching one sugar at a time

what are o glycans linked to

OH group on ser/ther

what are n glycan linked to

NH2 group on Asn. (most abundant glycoprotiens)

what is the consensus sequence of n linked glycoylation

ASN bind to any amino acid except pro then ser/thr

cotranslationally synthesized

N-linked glycoprotein

which is post-translationally synthesised

0 linked glycoprotiens

which chain is synthesized on the protein

o linked

which cain is added on en-block transfer of oligosachharide chain on protein

n linked