TLC - thin layer chromatography

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10 Terms

1
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The aim of chromatography is to…

separate a mixture into its constituents

2
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In this case the constituents are…

biological molecules

3
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2 key phases:

stationary and mobile

4
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Stationary phase:

chromatography paper or TLC plate

5
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In the stationary phase there is free…

OH- groups pointing outwards, in contact with mobile phase

6
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Mobile phase:

solvent

7
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The solvent is water for…

polar molecules

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The solvent is ethanol for…

non polar molecules

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The mobile phase flows…
carrying… with it

through and across the stationary phase, carrying biological molecules with it

10
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The distance moved by the constituents is based on their… and how well they…

size, how well they dissolve in solvent