* Louis Pasteur's experiment involved disproving spontaneous generation of microorganisms, which was the idea that microbes could arise from non-living matter.
* He used two flasks, one with a straight neck and one with a curved neck, both filled with nutrient broth. The straight-necked flask allowed dust and microbes to enter, while the curved-necked flask prevented them from reaching the broth.
* he broth in the straight-necked flask became cloudy with microbes, while the broth in the curved-necked flask remained clear, demonstrating that microorganisms did not arise spontaneously but came from outside sources.