Molecular Techniques (ASCPi)

PCR

An in vitro method that amplifies low levels of specific DNA sequences

Denaturation

Step in PCR in which the double stranded DNA is separated into single strands at 95C

18-30 bases

Primers are usually how long?

RT-PCR

It is useful in identification of HIV and Hepatitis C

Multiplex PCR

Uses numerous primers within a single reaction tube to amplify nucleic acid fragments from different targets

qPCR

Measures the amount of amplicons in real time since results can be seen at the end of each cycle

Ethidium bromide

In qPCR, which fluorescent dye is specific for double-stranded DNA?

SYBR Green

In qPCR, product labeling is currently done using a less toxic dye which is:

Probes

In qPCR, more specific detection of product is achieved with:

Southern blot

Blotting procedure in which DNA is denatured with restriction enzymes to create DNA fragments

Northern blot

Blotting procedure in which mRNA is separated by electrophoresis and blotted

Western blot

Blotting procedure in which proteins are separated electrophoretically, and identified through the use of labeled antibodies specific for the protein of interest