URINALYSIS: LEUKOCYTE ESTERASE

0.0(0)
studied byStudied by 0 people
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
Card Sorting

1/18

encourage image

There's no tags or description

Looks like no tags are added yet.

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

19 Terms

1
New cards

Leukocyte Esterase

  • It is used in the screening of urine culture specimens

  • a more standardized means for the detection of leukocytes

  • detects lysed leukocytes in alkaline urine

2
New cards

120 seconds

REAGENT STRIP: LEUKOCYTE ESTERASE

reaction time

3
New cards

Action of Leukocyte Esterase to catalyze the hydrolysis of acid ester

REAGENT STRIP: LEUKOCYTE ESTERASE

Principle [Strasinger]

4
New cards

Action of Leukocyte Esterase to cleave an ester and form an aromatic compound followed by an azo coupling reaction of the aromatic amine formed with a diazonium salt on the reagent pad. color produced from beige to violet

REAGENT STRIP: LEUKOCYTE ESTERASE

Principle [Brunzel]

5
New cards

Violet (Purple)

REAGENT STRIP: LEUKOCYTE ESTERASE

Color

6
New cards

derivatized pyrrole amino acid ester, diazonium salt

REAGENT STRIP: LEUKOCYTE ESTERASE

Reagents [Multistix]

7
New cards

indoxylcarbonic acid ester, diazonium salt

REAGENT STRIP: LEUKOCYTE ESTERASE

Reagents [Chemstrip]

8
New cards

5 - 15 WBCs/HPF (~11-36 WBCs/uL)

REAGENT STRIP: LEUKOCYTE ESTERASE

Sensitivity [Multistix]

9
New cards

10-25 WBCs/HPF (~10 WBCs/uL)

REAGENT STRIP: LEUKOCYTE ESTERASE

Sensitivity [Chemstrip]

10
New cards

  • Highly pigmented urine (phenazopyridine, nitrofuratonin)

  • Formalin

  • Strong oxidizing agents

REAGENT STRIP: LEUKOCYTE ESTERASE

False (+)

11
New cards

  • High SG

  • Strong oxidizing agents

  • Increased glucose (>3g/dL) or Protein (>500 mg/dL), High Conc. Ascorbic Acid

  • Gentamicin, Cephalosporin, Tetracycline

REAGENT STRIP: LEUKOCYTE ESTERASE

False (-)

12
New cards

Should be repeated on new specimen

REAGENT STRIP: LEUKOCYTE ESTERASE

Reporting: Trace

13
New cards

Small

REAGENT STRIP: LEUKOCYTE ESTERASE

Reporting: 1+

14
New cards

Moderate

REAGENT STRIP: LEUKOCYTE ESTERASE

Reporting: 2+

15
New cards

Large

REAGENT STRIP: LEUKOCYTE ESTERASE

Reporting: 3+

16
New cards

Oxalic acid

REAGENT STRIP: LEUKOCYTE ESTERASE

__ does not affect leukocyte esterase because it does not exist in urine with a pH greater than 4.5

17
New cards

False negative

REAGENT STRIP: LEUKOCYTE ESTERASE

But in Strasinger 7th Ed, Oxalic acid is considered ____

18
New cards

Lymphocyte

REAGENT STRIP: LEUKOCYTE ESTERASE

WBC with no Esterase

19
New cards

Neutrophil, Eosinophil, Basophil, Monocyte, Histiocyte, Trichomonas

REAGENT STRIP: LEUKOCYTE ESTERASE

With esterase