colorimeter
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6 Terms
1
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1
switch it on
2
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2
allow 15 mins for lamp to warm up and instrument to stabilise
3
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3
select correct colour filter
\
violet solution = yellow-green filter (&vv)
blue solution = yellow filter (&vv)
blue-green solution = red filter (&vv)
green solution = purple filter (&vv)
orange solution = green-blue filter (&vv)
purple-red solution = green filter (&vv)
\
violet solution = yellow-green filter (&vv)
blue solution = yellow filter (&vv)
blue-green solution = red filter (&vv)
green solution = purple filter (&vv)
orange solution = green-blue filter (&vv)
purple-red solution = green filter (&vv)
4
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4
insert blank cuvette and check reading is 0
5
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5
analyse your samples and check absorption several times throughout to check reproducibility
6
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inaccuracies may be due to
* incorrect use of cuvettes
* dirt / fingerprints / solution on outside
* condensation on cold samples (allow acclimitisation)
* air bubbles
* insufficient solution
* dirt / fingerprints / solution on outside
* condensation on cold samples (allow acclimitisation)
* air bubbles
* insufficient solution