Pet Blood Bank Seminar

Criteria for blood donor dogs (6)

Fit/healthy

1-8 years old

at least 25kg

Relaxed/confident temperment

Born and remained in UK/Ireland whole life

Not on long term medication

List the types of blood products (6)

Fresh/Stored Whole Blood

Packed Red Blood Cells

Fresh Frozen Plasma/ Frozen Plasma

Cryosupernatant

Cryoprecipitate

Platelet concentration

Shelf life of Fresh/Stored Whole Blood and components (6)

Fresh whole blood for 6 hours at room temperature, then stored in fridge for 21 days as stored whole blood

Contents:

• Erythrocytes

• Haemostatic proteins

• Plasma proteins

• Immunoglobulins

• Antiproteases

• Platelets
  

3 uses of Whole Blood

- haemostatic resuscitation
- haemostatic protein deficiency with blood loss
- Help arrest active haemorrhage in a patient with thrombocytopenia or thrombopathia

Shelf life and component of Packed Red Blood Cells

42-day shelf life

Erythrocytes only

2 uses of Packed Red Blood Cells

• anaemia

• haemostatic resuscitation (may be combined with fresh frozen plasma)

Shelf life and 4 components of Fresh Frozen Plasma

Stores for 1 year

Contents:

• Haemostatic proteins

• Antiproteases

• Immunoglobulins

• Plasma proteins

8 uses of  Fresh Frozen Plasma

• Disseminated intravascular coagulation

• Adder bite

• Consumptive coagulopathy

• Haemophilia (A and B)

• von Willebrand’s Factor Deficiency

• Angyostrongylus bleeding

• Acute haemorrhagic shock

Shelf life and 10 components of frozen plasma and cryosupernatant

FP = 5 years (including one year as fresh frozen plasma)
Cryo - 1 year

8 uses of frozen plasma and cryosupernatant

• Haemorrhagic gastroenteritis

• Hepatic coagulopathy

• Haemophilia B

• Hypoproteinaemia

• Resuscitative IVFT

• Immunoglobulin transfer

• Hypoalbuminaemia (cryosupernatant)

Shelf life and 5 components of cryoprecipitate

1 year

Contents = Factor I, VIII, XIII, xWF, fibronectin

3 uses of cryoprecipitate

Von Willebrand’s disease

Haemophilia A

Hypofibrinogenemia

Shelf life and components of Platelet concentrate

3 days

Platelets and some RBC (so typing is reccomonded)

2 uses of platelet concentrate

Uncontrolled/life-threatening haemorrhage due to thrombocytopenia/thrombocytopathia

Prophylactic treatment in patients with hereditary thrombopathia before surgery

What can happen following blood transfusion without typing the blood

Acute Haemolytic Transfusion Reaction due to intra/extravascular cell destruction

What is the most common blood type in dogs

DEA 1

Purpose of crossmatching over blood typing

The number of blood types is not known, so these tests directly determine if there will be a reaction between the donor and recipient

Explain the difference between major and minor cross matching and when would they be used?

Major - check recipient plasma for alloantibodies against donor erythrocytes

Minor - check donor plasma for alloantibodies against recipient erythrocytes. This can be used when using non-cellular products

What 2 reactions are seen when cross matching incompatible blood types?

Haemagglutination (clump)

Haemolysis (red plasma)

What are the blood types of cats and their reactivity patterns (4)

A- low amount of weak anti-B antibodies

B - high amount of anti-A antibodies

AB - no antibodies for either A or B antigen

Mik negative/positive