HARADA MORI CULTURE METHOD

Harada Mori Culture Method

This procedure is used to cultivate larvae stages of the hookworms and threadworm

Hookworm

Threadworm

Trichostrongylus spp.

When using Harada Mori technique, stool sample that is positive for what parasites?

Hookworm larvae

What will move downwards against the upward capillary movement of water and therefore can be recovered from the water and bottom of the tube

Strongyloides stercoralis larvae

This will move upwards and accumulate at the upper end of the filter paper

Filariform larvae (L3)

The infective stage, and caution must be observed in handling stool cultures

Necator americanus

Scientific name for New World Hookworm

Ancylostoma duodenale

Scientific name for Old World Hookworm

Ancylostoma caninum

Scientific name for Dog Hookworm

Ancylostoma braziliense

Scientific name for Cat Hookworm

Egg

Indistinguishable stage of Hookworm

Rhabditiform Larva (L1)

• Feeding stage of Hookworm

• Immature

Filariform larva (L3)

• Infective stage of Hookworm

• Mature

• Skin penetration

Filariform stage

Hookworms can be differentiated at what stage?

10 days

How many days does a harada mori culture method is kept in a dark room at room temparature?

15 minutes

How many minutes to immerse the test tubes in very hot water?

3-5 minutes

How many minutes to centrifuge the culture?

Low Power Field

what field is used when scanning the entire slide for presence of larva?

High Power field

what field is used when identifying of larva?

0.5g

In plastic bag method, how many amount of feces on one side of the filter paper leaving 4 cm at the pointed end and 2cm at the top end unsmeared

24-28 C

At what temperature should be used in incubated for plastic bag method