Recombinant DNA Technology Vocabulary

Flashcards about Recombinant DNA Technology

Biotechnology

A field of applied biology using living organisms and bioprocesses in engineering, technology, medicine, etc.

Biotechnology

The use of microorganisms.

Genetic engineering

Involves gene manipulations and modifications to move genes between different organisms.

Recombinant DNA

Genes from two different sources combined in vitro into the same molecule of DNA.

Genetic engineering

Alteration of the genetic constitution of cells/organisms by direct and selective modification/insertion/deletion of individual genes.

Recombinant DNA technology

Methodology for inserting a DNA fragment from one organism into the DNA of another organism for cloning the new recombinant DNA.

Gene cloning/DNA cloning/molecular cloning

Production of many identical copies of a DNA molecule by replication in a suitable host.

Plasmid

Vector to introduce gene of interest into host, often with antibiotic resistance markers.

Restriction enzyme

Enzyme used to cut plasmid and gene of interest at specific locations.

DNA ligase

Enzyme to join DNA pieces together to produce recombinant DNA.

Restriction enzymes

Cut DNA at specific nucleotide sequences.

Transformation

The process of transferring exogenous DNA into cells.

Multiple host systems

Used to produce recombinant human protein.

Gene delivery

A gene delivery method for generating induced Pluripotent Stem Cells (iPSC).

Cas9

An enzyme that cuts both strands of DNA