the microscope

EXPLAIN THE PROPER TECHNIQUE FOR TRANSPORTING THE MICROSCOPE.

WHEN TRANSPORTING THE MICROSCOPE, HOLD IT IN AN UPRIGHT POSITION WITH ONE HAND ON ITS ARM AND THE OTHER SUPPORTING ITS BASE. AVOID SWINGING THE INSTRUMENT DURING ITS TRANSPORT AND JARRING THE INSTRUMENT WHEN SETTING IT DOWN.

THE MICROSCOPE LENS MAY BE CLEANED (WITH ANY SOFT TISSUE).

FALSE; ONLY WITH SPECIAL GRIT-FREE LENS PAPER

THE MICROSCOPE SHOULD BE STORED WITH THE (OIL IMMERSION) LENS IN POSITION OVER THE STAGE.

FALSE; LOWEST-POWER OBJECTIVE

Ocular lenses

"EYEPIECES" ARE LOCATED ON TOP OF THE BODY TUBE AND USUALLY HAVE A MAGNIFICATION POWER OF 10X.

Body Tube

holds the ocular lens

Arm

supports upper parts and provides a carrying handle

Nosepiece

revolving device that holds objective lens

Objective Lens

adjustable lens system that permits the use of a scanning lens. low-power lens, higher power lens, oil immersion lens

Scanning objective

short objective lens and is used to scan the whole slide

Low-power objective

this is lens is longer than the scanning power objective lens and is used to view objects in greater detail

high-pwer objective

this lens will be the longest and is used to view an object in even greater detail

Stage

PLATFORM ON WHICH THE SLIDE RESTS FOR VIEWING

mechanical stage or spring clips

SECURE(S) THE SLIDE TO THE STAGE

coarse-adjustment knob

knob used to bring the object into approximate focus; used only with scanning power objective

fine-adjustment knob

SED FOR PRECISE FOCUSING ONCE INITIAL FOCUSING HAS BEEN DONE

condenser

DELIVERS A CONCENTRATED BEAM OF LIGHT TO THE SPECIMEN

iris diaphragm

USED TO INCREASE THE AMOUNT OF LIGHT PASSING THROUGH THE SPECIMEN

base

the flat surface of the microscope that rests on the table

WHEN BEGINNING TO FOCUS, USE THE (LOWEST-POWER) LENS.

true

WHEN FOCUSING, ALWAYS FOCUS (TOWARD) THE SPECIMEN.

F:FALSE; AWAY (SO YOU WONT GET TO CLOSE AND BREAK THE SLIDE)

A COVERSLIP SHOULD ALWAYS BE USED (WITH WET MOUNTS AND THE HIGH-POWER AND OIL LENSES).

F:FALSE; WITH WET MOUNTS only

movable nosepiece

carries the objective lenses; rotates so that the different objective lenses can be brought into position over the specimen

THE DISTANCE FROM THE BOTTOM OF THE OBJECTIVE LENS IN USE TO THE SPECIMEN IS CALLED THE

working distance

ASSUME THERE IS AN OBJECT ON THE LEFT SIDE OF THE FIELD THAT YOU WANT TO BRING TO THE CENTER (THAT IS, TOWARD THE APPARENT RIGHT). IN WHAT DIRECTION WOULD YOU MOVE YOUR SLIDE?

left

THAT AREA OF THE SPECIMEN SEEN WHEN LOOKING THROUGH THE MICROSCOPE IS THE

fiel

IF A MICROSCOPE HAS A 10X OCULAR AND THE TOTAL MAGNIFICATION AT THE PARTICULAR TIME IS 950X, THE OBJECTIVE LENS IS USE AT THE TIME IS

95X

WHY SHOULD THE LIGHT BE DIMMED WHEN LOOKING AT LIVING (NEARLY TRANSPARENT) CELLS?

TO PROVIDE MORE CONTRAST

IF, AFTER FOCUSING IN LOW POWER, ONLY THE FINE ADJUSTMENT NEED BE USED TO FOCUS THE SPECIMEN AT THE HIGHER POWERS, THE MICROSCOPE IS SAID TO BE

PARFOCAL

IF, WHEN USING A 10X OCULAR AND A 15X OBJECTIVE, THE FIELD SIZE IS 1.5 MM, THE APPROXIMATE FIELD SIZE WITH A 30X OBJECTIVE IS

.75MM

IF THE SIZE OF THE HIGH-POWER FIELD IS 1.2 MM, AND OBJECT THAT OCCUPIES APPROXIMATELY A THIRD OF THAT FIELD HAS AN ESTIMATED DIAMETER OF

.4MM

DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE?

RESOLUTION ___

INCREASE

DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE?

WORKING DISTANCE ___

DECREASE

DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE?

AMOUNT OF LIGHT NEEDED ___

INCREASE

DO THE FOLLOWING FACTORS INCREASE OR DECREASE AS ONE MOVES TO HIGHER MAGNIFICATIONS WITH THE MICROSCOPE?

DEPTH OF FIELD ____

DECREASE

DESCRIBE THE PROPER PROCEDURE FOR PREPARING A WET MOUNT.

THE OBJECT IS PLACED IN A DROP OF WATER (OR SALINE) ON A CLEAN SLIDE.
2. A COVER-SLIP IS HELD AT A 45 DEGREE ANGLE WITH THE FINGERTIPS.
3. IT IS LOWERED CAREFULLY OVER THE WATER AND THE OBJECT.