10 proof of semi conservative replication
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14 Terms
what did watson and crick discover?
the structure of DNA using rosalind franklins research on x ray diffraction
what did watson and crick hypothesise?
that DNA replicates conservatively or semi conservatively
what semi conservative replication?
each replicated DNA molecule contains one of the original DNA strands and one new synthesised DNA strand
what’s conservative replication?
the original DNA remains intact following DNA replication and the two newly synthesised strands of DNA join together
who conducted an experiment to find whether DNA was conservative or semi conservative?
meselson and stahl
what did meselson and stahls experiment prove?
DNA replication must be semi conservative
what are DNA bases?
nitrogenous (contain nitrogen)
how many isotopes does nitrogen have?
2: 14N and 15N
which isotope of nitrogen is heavier?
15N
what do bacteria do with nitrogen?
they take in nitrogen isotopes to make new DNA nucleotides
what happens to bacteria grown in a medium containing 14N?
will have DNA which only contains this isotope and be lighter
what happens to bacteria grown in a medium containing 15N?
will have DNA only containing this isotope and be heavier
what are the results of DNA samples being spun in a centrifuge?
15N - will settle lower down as it’s more dense
14N - will settle higher up as it’s less dense