Bio exam 2

Buffers control the pH at which the reaction occurs. ________

Purpose of reaction buffers.

Trypsin's activity significantly decreases when the buffer's pH lowers from pH 8 to ________.

pH 5.

Trypsin is a protease that breaks peptide bonds in dietary proteins, producing ________ and amino acids.

small peptides

If TCA is not added, the absorbance would be _________.

unusually high.

Enzymes may change shape during __________ binding.

substrate

A p-value below 0.05 indicates ________ differences between groups.

significant

Mixing 50 µL of reagent with 950 µL water creates a ________ dilution.

1:20

Enzymes are __________ that speed up reactions.

proteins

What happens if the substrate is not added? The absorbance would match __________.

negative controls.

To stop the enzyme reaction, one must add __________.

TCA.

Real-world behavior of trypsin: It is secreted as trypsinogen to prevent digestion of __________ proteins.

pancreatic.

For statistical analysis, the standard deviation reflects __________ among results for experimental conditions.

variability.

Analyze graphs to determine the point of highest enzyme __________.

activity.

The optimal temperature or pH for enzymes can be found by analyzing __________.

graphs.

Look for __________ in enzyme activity curves to determine optimal conditions.

peaks.

Independent variables may include ________ or temperature.

salinity.

TCA addition in enzyme assays serves to __________ enzyme activity after the reaction period.

stop.