1st Prelims: Blood Components: Preparation, Storage, and Shipment

Blood collected from donors contains all cellular and liquid elements

whole blood

What are the parts of blood used for treating patient?

1. RBCS

2. Plasma

3. Platelest

4. Cryoprecipitated antihemophiliac factor

Use blood or blood components to treat a disease in a patient

Hemotherapy

Blood Collection Bag has up to ___ satellite bags attached.

four

a closed system consisting of main bag with needle, tubing, and up to four satellite bags attached

blood collection bag

Standard phlebotomy

450 mL ± 45 mL or 500 mL ± 50 mL

Anticoagulant Preservative Solutions

• Standard volume:

1. 63 mL for 450 mL collections

2. 70 mL for 500 mL collections

Used in ATP synthesis

Adenine

Chelates calcium to prevent coagulation

Citrate

CPD

Citrate-phosphate-dextrose

CP2D

Citrate-phosphate-2-dextrose

CPDA-1

Citrate-phosphate dextrose adenine-1

Sugar to support RBC life

Dextrose

Buffer to prevent decreased pH

Sodium biphosphate

These decrease as RBCs are stored

1. Glucose,

2. ATP, 2,3-BPG,

3. pH

After cells are transfused, these are restored in about 24 hours

1. ATP

2. 2,3-BPG levels

Substances that increase during storage are all ?

metabolic end products

Substances that increase during storage are all metabolic end products such as

Potassium, hydrogen ions, etc.

Additive solutions

○ AS-1 contains =

○ AS-3 contains =

○ AS-5 contains =

○ AS-1 contains = mannitol.

○ AS-3 contains = citrate andphosphate.

○ AS-5 contains = mannitol.

Additive solutions

• These must be added within ____ of collection

Additives

• extend the shelf life to ____ and

• reduce RBC____ during transfusion

• 72 hours

• 42 days

• viscosity

Rejuvenation solution

• Contains

1. phosphate,

2. inosine,

3. pyruvate

4. adenine

Its purpose is to restore: 2,3-BPG and ATP levels, before freezing or transfusing a unit, and it may necessary for autologous or rare units.

Rejuvenation solution

RBCs can be rejuvenated up to ____ past the expiration date or be frozen for future use.

3 days

RBCs can be rejuvenated, stored up to ___ hours at ___ C, and transfused.

24 ; 1-6°

If platelets are to be prepared from whole blood, ____ are required

two spins

Plasma is separated into ___ bag, leaving platelets with ___ of plasma in platelet bag.

• FFP

• 40 to 70 mL

Plasma is either frozen to make FFP within ____ of collection or frozen and later thawed in refrigerated conditions to make: ____, _____

8 hours

• cryoprecipitate

• cryo-poor plasma.

Whole blood storage and expiration

• 1-6°C

• With CPD

  • CP2D anticoagulants - 21 days

  • CPDA-1 anticoagulant - 35 days

  • Adsol (AS-1, AS-3, orAS-5) - 42 days

RBCs storage and expiration

• 1-6°C

• With CPD

  • CP2D anticoagulants - 21 days

  • CPDA-1 anticoagulant - 35 days

  • Adsol (AS-1, AS-3, orAS-5) - 42 days

Platelets storage and expiration

• 20-24°C with rotation

• 5 days

FFP storage and expiration

• -18°C, -65°C,

• 1 year, 7 years

Cryoprecipitate storage and expiration

• -18°C

• 1 year

RBC frozen storage and expiration

• -65°C

• 10 years

RBCs (deglycerolized, washed) storage and expiration

• 1-6°C

• 24 hours after thawing (deglycerolization)

RBCs (irradiated) storage and expiration

• 1-6°C

• 28 days or on originally assogned outdate, whichever comes first

Platelest (pooled) storage and expiration

• 1-6°C

• 4 hours after pooling

Cryoprecipitate (pooled) storage and expiration

• 1-6°C

• 4 hours after pooling

FFP (thawed )storage and expiration

• 1-6°C

• 24 hours

Plateletpheresis storage and expiration

• 20-24°C

• 5 days

Garanulocytepheresis storage and expiration

• 20-24°C

• 24 hours

All refrigerators, freezers, and platelet incubators must have recording devices that monitor the temperature at least every ____

4 hours

Temperature for RBCs of ____ is required during transport.

1-10°C

The ice used in transporting RBC is double-bagged and weighs approximately ___ pounds

nine

Frozen components are shipped on ___. These should be well wrapped because dry ice evaporates, and space in the box for movement should be allowed.

dry ice

Platelets are shipped at ____, they can survive without agitation for a maximum of ____.

• room temperature

• 24 hours

Only ___ should be infused with blood components

normal saline

A standard _____ filter must be used with all blood components

170-micron

____ may be used to reduce the number of leukocytes transfused with RBCs

Leukoreduction filters

The maximum transfusion time allowed for ___ unit to be transfused is 2-4 hours.

one

Parts of blood used for treating patients

Components