Polishing in Downstream Processing

Vocabulary flashcards focusing on key terms and definitions related to polishing and drying techniques in downstream processing.

Polishing

The final step in downstream processing, critical for achieving desired purity, potency, and yield of the final product.

Cell Harvesting

Techniques used to separate and collect cells from a culture, including centrifugation and filtration methods.

Centrifugation

A process of separating suspended particles from a liquid medium by spinning them at high speed.

Microfiltration

A filtration method that uses membranes with pore sizes to separate microorganisms and suspended solids from liquids.

Ultrafiltration

A pressure-driven membrane filtration process that separates substances in a liquid based on size.

Cell Disruption

Methods such as homogenization and bead milling used to break open cells to release their contents.

Extracellular Products

Products secreted by cells into the surrounding environment during culture or fermentation.

Drying Techniques

Various methods used to remove moisture from products, including spray-drying, freeze-drying, and tray-drying.

Spray Drying

A technique that involves atomizing a liquid solution into droplets and drying them in a hot air stream.

Freeze Drying

Also known as lyophilization; a process that removes moisture from frozen products by sublimation under vacuum.

Tray Drying

A method of drying where product solutions are spread on trays and heated in a hot air oven.

Bioreactor

A vessel or container in which biological reactions take place, commonly used in fermentation and cell culture.

Critical Moisture Content

The moisture level at which the drying rate begins to decline significantly due to changes in the material's properties.

Final Purification Stages

Processes involved in achieving high purity of a product, including chromatography and electrophoresis.

Shelf Life

The period during which a product remains usable and retains its intended quality and efficacy.

Process Parameters in Drying

Factors such as temperature, pressure, residence time, humidity, and airflow that affect the drying process.