Microscopes and Magnification

What is the max resolution?

0.2 um

What can electron microscopes be used to observe?

smaller organisms

Microscope

What are microscopes used to analyse and observe?

cell components and organelles

Objective lens

x4
x10
x40
x100

What does magnification tell you?

how many times bigger the image produced by the microscope is than the real-life object you are viewing

What is resolution?

the ability to distinguish between objects that are close together

The higher the resolution of an image

the greater the detail that can be seen

Magnification formula

image size = actual x magnification

What can cells and organelles be measured with?

an eyepiece graticule

What is the eyepiece graticule?

transparent

What does an eyepiece graticule not have any?

units (arbitrary units)

What is only seen?

the scale with major and minor divisions at equal increments

cm to mm

x10

mm to um

x1000

um to nm

x1000

nm to um

/1000

um to mm

/1000

mm to cm

/10

Light microscopy

uses light to form an image

What can light microscopes be used to view?

living specimens in their natural colours

What do light microscopes use to magnify images?

glass lenses to bend light

What is resolution limited by?

the wavelength of the radiation

What are they used to observe?

eukaryotic cells

What can't they be used to observe?

smaller organisms such as ribosomes

What is the max magnification?

x1500

Size

small and easy to carry

Vacuum need?

no

What is the sample preparation?

simple

What can the specimens be?

living or dead

Electron microscopes

uses electrons to form an image

What can electron microscopes generate?

images at a much higher magnification and resolution

What can't electron microscopes view?

living specimens in natural color

What images do electron microscopes produce?

monochromatic

What do electron microscopes increase?

the resolution

What is the image produced?

detailed due to the electrons shorter wavelength

What is the max resolution?

0.0002um

What is the max magnification?

x1,500,000

Size

large and an instillation (can't be moved)

Vacuum needed?

yes

What is the sample preparation?

complicated

What do the specimens have to be?

dead