Zoonosis/Legionella/Miscellaneous fastidious gram negative rods

Which of the following amino acids are required for growth of Francisella tularensis?

A.Leucine and ornithine

B.Arginine and lysine

C. Cysteine and cystine

D.Histidine and tryptophan

C. Cysteine and cystine

In suspected cases of brucellosis, the optimal specimen to be collected for the isolation of the etiologic agent is

A. Blood

B. Urine

C. Cerebrospinal fluid

D. Nasopharyngeal exudates

A. Blood

In case of legionellosis

A.Person-to-person transmission is common

B.Farm animals are important animal reseroirs

C.Patients can present with a self-limited nonpneumonic febrile illness

D.Specimens may be cold enriched to enhance recovery of the organism

C.Patients can present with a self-limited nonpneumonic febrile illness

A 29-year-old male who often hunted rabbits and spent a lot of time in the woods was admitted to the hospital with skin ulcers on his upper extremities. At 48 hours, a small coccobacillus was recovered from the aerobic blood culture bottle only. The organism stained poorly with Gram stain, but did stain with acridine orange. Cultures taken from the ulcers did not grow on primary media. What is the most likely identification?

A.Pseudomonas aeruginosa

B.Pseudomonas fluorescens

C.Chryseobacterium spp.

D.Francisella tularensis

D.Francisella tularensis

Which of the following statements is not true of Brucella?

A.Infection may occur via abrasions of the oral mucosa, conjunctiva, and genitals

B.They are fastidious and require supplemented media for isolation

C.The risk of accidental laboratory infection is no greater than with any other organism

D.Phage and dye sensitivity tests are used for identification to the species level

C.The risk of accidental laboratory infection is no greater than with any other organism

Which of the following is not true about Pasteurella multocida?

A.Most common human infections occur in soft tissues, bones, and joints

B.Humans harbor the organism as part of their normal flora

C.It is the most virulent of the species in the genus

D.It grows on sheep blood agar

B.Humans harbor the organism as part of their normal flora

Which of the following Legionella spp. is positive for Hippurate hydrolysis?

A.L. micdadei

B.L. longbeachae

C.L. gormanii

D.L. pneumophila

D.L. pneumophila

Which of the following is the optimal clinical specimen for the recovery of Legionella pneumophilia?

A.Stool

B.Blood

C.Bronchial washings

D.Nasopharyngeal swab

C.Bronchial washings

A suspected case of Legionnaires' disease was noted on the request form for a culture and sensitivity ordered on a sputum sample. The patient was a 70-year-old male who presented with a positive serological test for Legionella spp. What is the most efficient way to confirm the infection using the submitted sample?

A.Culture the sputum on MacConkey agar

B.Gram stain of the sputum

C.Acid-fast staining

D.Direct immunofluorescent microscopy

D.Direct immunofluorescent microscopy

Which medium is recommended for the recovery of Brucella spp. from blood and bone marrow specimens?

A.Biphasic Castenada bottles with Brucella broth

B.Blood culture bottles with Brucella broth

C.Bordet-Gengou agar plates and THIO broth

D.Blood culture bottles with THIO broth

A.Biphasic Castenada bottles with Brucella broth

Buffered charcoal yeast extract agar is the recommended medium for the recovery of

A.Hafnia alvei

B.Legionella pneumophilia

C.Neisseria meningitidis

D.Vibrio cholerae

B.Legionella pneumophilia

Which of the following species of Pasteurella is associated with human infections following cat bites?

A.P. avium

B.P. canis

C.P. multocida

D.P. stomatis

C.P. multocida

An elderly woman who cared for several domestic cats was hospitalized with suspected cat-scratch disease (CSD). Blood cultures appeared negative, but a small, slightly curved pleomorphic gram-negative bacillus grew on BHI agar (brain, heart infusion agar with 5% horse or rabbit blood). What is the most likely identification?

A.Bartonella spp

B.Brucella spp

C.Kingella spp

D.Haemophilus spp

A. Bartonella spp

Which media should be used to recover Bordetella pertussis from a nasopharyngeal specimen?

A.Chocolate agar

B.Blood agar

C.MacConkey agar

D.Bordet-Gengou agar

D.Bordet-Gengou agar

Legionella pneumophila is the etiologic agent of both Legionnaires disease and

A.Swine fever

B.Pontiac fever

C.Rift Valley fever

D.San Joaquin Valley fever

B.Pontiac fever

Brucella spp. are

A.The etiologic agents of relapsing fever

B.Small spiral organisms

C.Primarily a cause of endogenous human infections

D.Intracellular pathogens

D.Intracellular pathogens

Legionella pneumophila

A.Infections are most often acquired from environmental sources

B.Metabolizes a number of carbohydrates

C.Stains easily on the routine Gram stain

D.Does not autofluoresce

A.Infections are most often acquired from environmental sources

In addition to CO2 requirements and biochemical characteristics, Brucella melitensis and Brucella abortus are differentiated by growth on media containing which two dyes?

A.Basic fuchsin and thionin

B.Methylene blue and crystal violet

C.Carbol fuchsin and iodine

D.Safranin and methylene blue

A.Basic fuchsin and thionin

Foodborne outbreaks of brucellosis are most commonly associated with eating

A.Raw shellfish

B.Imported cheese

C.Contaminated potato salad

D.Improperly cooked hamburger

B.Imported cheese

Which medium is best for recovery of Legionella pneumophila from clinical specimens?

A.Chocolate agar.

B.Bordet-Gengou agar

C.New yeast extract agar

D.Buffered charcoal-yeast extract (CYE) agar

D.Buffered charcoal-yeast extract (CYE) agar

A 5-year-old nonimmunized male with a persistent cough, fever, and flulike symptoms was admitted to the hospital. Nasopharyngeal swabs were cultured on 15% blood, chocolate, Bordet-Genjou, and Regan-Lowe (with 10% charcoal) agars. All media grew a gram-negative coccobacillus. Carbohydrate and biochemical tests were negative. What is the most likely identification?

A.Haemophilus influenza

B.Bordetella pertussis

C.Haemophilus parainfluenzae

D.Bordetella bronchiseptica

B.Bordetella pertussis

Smooth gray colonies showing no hemolysis are recovered from an infected cat scratch on blood and chocolate agar but fail to grow on MacConkey agar. The organisms are gram-negative pleomorphic rods that are both catalase and oxidase positive and strongly indole positive. The most likely organism is:

A.Capnocytophaga spp.

B.Pasteurella spp.

C.Proteus spp.

D.Pseudomonas spp.

B.Pasteurella spp.

Which microorganism will grow only on culture media supplemented with either cysteine or cystine?

A.Actinobacillus lignieresii

B.Bartonella bacilliformis

C.Francisella tularensis

D.Kingella kingae

C.Francisella tularensis