MOL-BIO LAB - COVID 19 DETECTION

Sample collection
Nucleic Extraction
Nucleic acid amplification
Detection

Steps of Molecular Diagnostics

Area A

Cleanest room

Area B

Lyses of specimen is done

Area A: PCR Reagent MIX
Area B: Nucleic Acid Extraction
Area C: PCR Amplification
Area D: Post-Amplification

Molecular Laboratory Working Areas

2-8

At what temp do you store extracted RNA while preparting the buffer mix

Cell Lysis
Separation of DNA
Alcohol Precipitaion
Wash
Ellusion buffer

Step in Nucleic Acid Extraction Methods

Column-Based Extraction

Mostly used extraction methods

Magnetic Bead-Based Extraction

Extraction used for gene sequencing

Organic extraction
Inorganic extraction

Liquid-phase extraction methods include

Request Form
Swab
Leakage
Label
Color of media - Pink to orange

In sample collection you should see these:

2-8C

Sample should be refrigerated at this temp

-70C

if the speciment for COVID 19 will be tested next week the temp for storage is

-20C

Reagents should be stored at this temp