Receptor proteins relay messages, transport proteins move things in and out
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Which is faster: carrier proteins or channel proteins in the cell membrane?
Channel proteins
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What does cholesterol do in the cell membrane?
Cholesterol maintains fluidity by protecting against temperature fluctuations
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What do the cell membrane's aquaporins do?
Allow water to diffuse in
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What is diffusion?
The natural spreading of molecules from high to low concentrations
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What is passive transport?
Molecules that are charged/polar need protection from the hydrophobic heads, so they travel through protein channels
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What is facilitated diffusion?
Molecules that are charged/polar need protection from the hydrophobic heads, so they travel through protein channels
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What is active transport?
Movement of molecules against the gradient through protein pumps, using ATP energy
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What is the element ratio in carbohydrates?
C:H2O
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What is the element ratio in proteins?
CHON, variable
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What is the element ratio in lipids?
C/1H2O
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What is the element ratio in nucleic acids?
CHONP, variable
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What is the monomer of carbohydrates?
Monosaccharides
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What is the monomer of proteins?
Amino acids
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What is the monomer of lipids?
Glycerol; fatty acids
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What is the monomer of nucleic acids?
Nucleotides; ribose (RNA)/deoxyribose (DNA);
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What is the shape/structure of a carbohydrate?
Ring shape
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What is the shape/structure of a protein?
Variable
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What is the shape/structure of a triglyceride?
Chain shape: glycerol backbone with three fatty acids/carboxylic acids
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What is the shape/structure of a phospholipid?
'Head-and-tail' shape: Hydrophilic head of a phosphate attached to a glycerol; hydrophobic tails of two saturated fatty acids attached to the glycerol, one of which turns into a "kinked" unsaturated fatty acid
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What is the shape/structure of a nucleic acid?
RNA: single helix; DNA: double helix
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What is the purpose of a carbohydrate?
for quick energy (glucose), energy storage (starch, fructose), building molecules (ribose), structure (chitin)
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What is the purpose of a protein?
Antibodies, enzymes, messengers, structure, almost all functions
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What is the purpose of a lipid?
Energy storage; warmth; cell membrane
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What is the purpose of a nucleic acid?
Storage of genetic information (DNA); transporting information from DNA to ribosome (RNA)
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Name and describe the cell cycle phases.
G1 (cell contents (not chromosomes) duplicated)
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S (chromosomes duplicated)
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G2 (cell grows; chromosomes double-checked)
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Mitosis
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Cytokinesis
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How is the cell cycle regulated?
By cyclins and CDK
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What are the steps for DNA replication?
Gyrase relaxes the DNA
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Helicase unwinds the DNA
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DNA polymerase takes free-floating nucleotides and attaches them to the now-exposed base pair on the mother strand
Metaphase (Chro's line up at the metaphase plate) Anaphase (Chromosomes separate, driven by motor proteins)
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What is the mitotic spindle made out of?
2 asters (the T) and kinetochore tubules, which connect at the chromosome's centromere
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Where does glycolysis occur?
In the cytoplasm.
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What does glycolysis do?
Breaks down glycolysis into pyruvate and two molecules of ATP
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What is metabolism?
The chemical processes that occur within a living organism in order to maintain life.
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What is the purpose of cellular respiration?
To break down carbohydrates into ATP molecules.
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What are the three stages of cellular respiration?
1. Glycolysis
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2. Krebs cycle
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3. Electron transport chain
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What are the steps of photosynthesis?
1. Light hits the thylakoid membrane, splitting H2O into O2 and releasing electrons
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2. The electrons travel down to special enzymes and generate ATP and NADPH from NADP and ADP
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3. Calvin cycle
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What do enzymes do?
Catalyze reactions
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What are enzymes made out of?
Proteins
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What is the 'active site'?
The area of an enzyme where the reaction occurs.
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How many molecules can one enzyme react with?
One; enzymes are specific
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Do enzymes change shape to fit the molecule?
Yes
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What do you call the molecule that goes into an enzyme?
The substrate
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What is a competitive inhibitor?
A substrate that binds to the active site of an enzyme and prevents it from working
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What is a non-competitive inhibitor?
A substrate that binds to a different site on the enzyme and changes its shape
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What does tRNA do?
Transfer amino acids to the ribosome
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What is a codon?
A set of 3 nucleotides on DNA that code for one amino acid
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What is transcription?
The transfer of DNA to an mRNA strand
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What is translation?
Changing the information on an mRNA strand to an amino acid chain via a ribosome
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Where does mRNA leave the nucleus?
Through nuclear pores
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What is an anticodon?
A set of 3 nucleotides that correspond to one amino acid
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How do histones affect gene regulation?
They control what genes can be accessed by coiling around DNA.
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What is gene regulation?
Regulating how traits are expressed through a variety of mechanisms.
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What are 'enhancer' sites?
Sites on DNA that increase the rate of transcription of a certain gene.
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What are 'silencer' sites?
Factors that slow the rate of transcription of a gene.
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What are some methods of gene regulation?
Regulators on the rate of transcription, the processing of RNA molecules, the stability of RNA molecules, and the rate of translation.
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What are introns?
Parts of DNA that do not code for an amino acid
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What do introns do?
Regulate gene expression
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Missense mutation
Replaces one base pair and produces a different amino acids
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Nonsense mutation
Replaces one base pair and produces a premature stop codon
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Insertion mutation
An extra base pair inserted where it is not called for
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Deletion mutation
Deletion of a base pair
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Duplication mutation
A piece of DNA that is copied once or multiple time
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Frameshift mutation
A shift in the DNA reading frame; i.e.
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CGA TAG CAG ACG becomes
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GAT AGC AGA CG
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Mendel's 1st law (Segregation)
The two members of a gene pair (alleles) segregate from each other in the formation of gametes. Half the gametes carry one allele, and the other half carry the other allele.
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Mendel's 2nd law (Independent assortment)
Genes for different traits assort independently of one another in the formation of gametes.
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What does a "3:1" phenotypic ratio mean?
Three of four organisms will have one phenotype, and one of four will have another phenotype when two heterozygous organisms are crossed.
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What is the F1 generation?
The first generation out from the parent generation.
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What is the F2 generation?
The generation that results when the F1 generation is all bred together.
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Which does NOT follow Mendel's laws: genotypes or phenotypes?
Phenotypes
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What ratio do lethal genotypes change; genotype or phenotype?
Phenotype; because we don't see the phenotype as the organism dies quickly, we assume the phenotypes are all dominant