Video: Microbiological Laboratory Safety Issues - Chapter 9

Flashcards covering key concepts from the microbiological laboratory safety notes, including safety organizations, biosafety levels, chemical safety, radiation, PPE, and safety considerations in healthcare settings.

There are guidelines and standards available to all microbiology laboratories which were established by three organizations in the U.S.: __.

OSHA, CDC, and WHO

OSHA was established in __.

1971

CDC is a major component of the US Department of Health and Human Services __.

HHS

WHO is established by __.

the United Nations

Hand Hygiene guidelines were developed by __, SHEA, APIC and IDSA.

HICPAC

The Biosafety Program has four components: LBNL Biosafety Manual; Online Exposure Control Plan; Bloodborne training program; and __ training program.

Biosafety

NIH created risk groups to classify agents into __ categories.

four

BSL-4 agents include Ebola virus, Variola virus (smallpox), and __.

Marburg virus

BSL-2 includes infectious agents of moderate risk with ingestion or mucous membrane transmission and corresponds to __.

BSL-2

BSL-1 comprises low-risk agents not known to cause human disease, such as __.

E. coli

Sulfuric acid has synonyms including __.

Battery acid

Sulfuric acid exposure limits are NIOSH REL and OSHA PEL, both TWA __ mg/m³.

1

Sulfuric acid is a colorless to dark-brown, oily, __ liquid.

odorless

Sulfuric acid reacts violently with __ with evolution of heat.

water

IDLH for sulfuric acid is __ mg/m³.

15

First aid for sulfuric acid exposure includes eye irrigation, skin washing, and respiratory support; treat with __.

medical attention immediately

Every laboratory should have a , guidelines for labeling chemical containers, and MSDSs for each chemical.

Chemical Hygiene Plan

Radiation safety requires shielding and/or wear devices, and exposure may be a cumulative process.

monitoring

Noise hazards in the lab can come from pumps and ultrasonicators; damage is __ and irreversible.

cumulative

Many labs use ultra-cold freezers or __ to preserve samples.

liquid nitrogen

Fire extinguishers must be inspected at least every __ months.

12

Multipurpose fire extinguishers are effective against type A, B, and __ fires.

C

Perchloric acid hoods must be used with procedures using __.

perchloric acid

Autoclaves use superheated steam under pressure; most transmissible agents are eliminated except __ and prions.

strain 121 archaea

Eyewashes and safety showers must be in reach within __ minutes of exposure.

10

Waste disposal containers include biohazardous wastes and __ containers.

sharps

PPE in the lab includes gloves, respiratory protection, laboratory coats, and __ protection.

eye

Physician offices and clinics are regulated by OSHA and should have management procedures for __ training.

blood-borne pathogen

Hospitals must have a hospital safety program with policies for __.

industrial hygiene

Nursing homes require ergonomic guidelines to prevent __ disorders.

musculoskeletal

Standards include hazardous waste __ and emergency response.

operations

Home safety is a concern with the increase in home-based __ care.

hospice