LEC DNA TECHNOLOGY

recombinant dna

term used to describe the combination of two dna strands that are constructed artificially

genetically modified organism

An organism that has undergone a recombinant DNA procedure

cloning

Genetically modified copy of an organism, and it may

be naturally occurring or created in the laboratory

stem cell

Cells with the potential to develop into many different

types of cells in the body

embryonic and adult stem cells

2 main types of stem cells

stem cell

serves as a repair system for the body

unspecified and undifferentiated

pluripotent stem cell

Are descendants of totipotent cells and can

differentiate into nearly all cells

embryonic cells

pluripotent cells give rise to ____

pluripotent stem cell

cells that are derived from any of the 3 germ layers

unipotent stem cell

As a pluripotent cell type, a human

embryonic stem cell is capable of forming all

adult cell types

unipotent stem cell

stem cell that is specific to one type of cell

totipotent stem cell

where all cells come from and can differentiate into embryonic and extraembryonic cell types

totipotent stem cell

these cells are produced from the fusion of an egg and sperm cell

multipotent stem cell

Can differentiate into a number of cell types, but only those of a closely related family of cells

amplicons

product of pcr

denaturation, annealing, elongation

steps of pcr

94 to 96 degrees celsius

temperature of denaturation step

-68 degrees celsius

temperature of annealing step

72 degrees celsius

temperature of elongation step

dna fingerprint technology

laboratory technique used to establish a link between biological evidence and a suspect in criminal investigation

southern blotting

technique used in dna fingerprint processing