lec 10.4 - lambda integration

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7 Terms

1
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final step of lambda infection?

  • integration

  • To be fully lysogenic the viral genetic material needs to integrate into
    the host’s chromosome (lambda DNA needs to integrate into bacterial genome)

2
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where does integration into E.coli chromo happen?

  • Integration happens by site-specific non-
    homologous recombination when lambda integrates between the Biotin (bio) and Gal genes on the E. coli chromosome

  • Lambda and E.coli chromosome line up at a specific site that has homology: POP’ in lambda and BOB’ on chromosome - very small location between the Bio
    and Gal genes on the E. coli chromosome

<ul><li><p>Integration happens by site-specific non-<br>homologous recombination when lambda integrates between the Biotin (bio) and Gal genes on the E. coli chromosome</p></li><li><p>Lambda and E.coli chromosome line up at a specific site that has homology: POP’ in lambda and BOB’ on chromosome - very small location between the Bio<br>and Gal genes on the E. coli chromosome</p></li></ul>
3
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what are the genes involved in lambda integration?

  • POP’ (attP)

  • BOB’ (attB)

  • both get restored after exision

<ul><li><p>POP’ (attP)</p></li><li><p>BOB’ (attB)</p></li><li><p>both get restored after exision</p></li></ul>
4
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what proteins are involved in integration? and what do they do?

  • Int: inserts into bacteria

  • Xis: excision - will excise lambda back out later

  • IFH: integration host factor - inhibits the host from getting rid of lambda DNA

<ul><li><p>Int: inserts into bacteria</p></li><li><p>Xis: excision - will excise lambda back out later</p></li><li><p>IFH: integration host factor - inhibits the host from getting rid of lambda DNA</p></li></ul>
5
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what are prophages?

product of lambda and bacterial chromosome integration

6
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how do prophages turn back into lytic phages?

  • when cI gets cleaved or the stability of cII is compromised

  • when cI gets cleaved it cant bind to DNA

<ul><li><p>when cI gets cleaved or the stability of cII is compromised</p></li><li><p>when cI gets cleaved it cant bind to DNA</p></li></ul>
7
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what cleaves cI?

UV light (from environment) and RecA (from the cell)

  • both lead to induction to lytic cycle