Comprehensive Table of Vectors and Their Properties

These flashcards cover important vectors used in molecular biology, detailing their properties, functions, and selection markers.

pBR322

A general cloning vector with an insert size of ~0.3–7 kb, that uses ampicillin and tetracycline as selection markers.

pUC19

A high-copy-number cloning vector with a size of ~0.1–10 kb, that includes a lacZ gene for blue-white screening.

pBluescript II

A cloning and sequencing vector, approximately ~0.1–10 kb, that utilizes ampicillin as a selection marker.

pGEM-T

A T/A cloning vector for PCR products, with an insert size of ~0.1–3 kb and ampicillin as a selection marker.

pET Series

A plasmid series for high-level protein expression in E. coli, utilizing a T7 promoter.

pBAD Series

An expression vector with tight control over gene expression in E. coli, derived from pBR322.

pCMV Series

A mammalian expression vector utilizing the cytomegalovirus promoter, generally sized ~0.5–10 kb.

pLenti Series

Lentiviral vectors that allow gene delivery into dividing and non-dividing cells.

pAdEasy System

An adenoviral vector system designed for high-level transient gene expression.

BAC (Bacterial Artificial Chromosome)

A cloning vector used for large DNA fragments, sized ~100–300 kb, with chloramphenicol as a selection marker.

YAC (Yeast Artificial Chromosome)

A cloning vector for large genomic fragments in yeast, typically sized ~100–2000 kb.

Cosmids

Vectors typically sized ~30–45 kb, used for large DNA fragment cloning by combining properties of plasmids and phages.

Lambda Phage Vectors

Bacteriophage vectors used for cDNA and genomic DNA libraries, with an insert size varying from ~9–23 kb.

pZ plasmids

Minimal plasmids with reduced sequences sized ~0.1–3 kb, providing stability in cloning.