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What is an important reason why we will need to know about pregnancy detection in production management?
-for management issue- controlled breeding season, repeat breeders
In dairy small ruminants, there is a transitional period. What is produced by the dam during this period?
colostrum
What are the FOUR cardinal signs of pregnancy?
- amnionic slip
- chorioallatoic membrane slip (on rectal palp)
- placentomes
- fetus
What species has oblong/circular placentomes?
cattle
What species has C shaped placentomes?
sheep and goats
T/F You can use observation, physical exam, or diagnostics to detect if there is a pregnancy
True
What are FOUR observations that can be made for diagnosis of pregnancy?
- abdominal contour
- mammary development
- Springing
- Failure to return to estrus
Where does the abdominal contour begin when a small ruminant is pregnant?
Why?
lower right abdomen- this is where the uterus lie (because the rumen is on the left side)
T/F If you us mammary development to determine pregnancy, you need to be aware that there is false positives
True
What are TWO things that can cause the mammary gland to develop that is NOT pregnancy?
- inappropriate lactation syndrom
-pseudopregnancy
What can lead using Springing for pregnancy diagnosis to be misleading?
fat deposition
What TWO species can you use rectal palpation on to determine pregnancy?
- Cattle
• Large camelids
What technique can be used to determined if small animals or small ruminants are pregnant?
Ballottement in the lower right quadrant/flank
What is Dr. Water's preferred method of ballottement?
Midline just cranial to udder
What are the THREE avaliable diagnostic devices that can be used for pregnancy?
• Ultrasound
• Radiographs
• Hormone/enzyme assay
What are the THREE types of ultrasound?
• A mode
• B mode
• Doppler
What ultrasound is the simplest because it uses a single transducer that scans a line through the tissues with the echoes plotted on the screen as a function of depth and detect fluid filled organs (10-20 cm depth)?
A mode
What can cause a false positive with an A mode U/S? (2)
•Hydrometra or pyometra
• Urinary Bladder
What can cause a false negative with the A mode U/S? Why?
Late gestation- Fluid in uterus has diminished relative to the size of the fetus
What A mode U/S has intermittent beep when sufficient skin contact
occurs but needs oil, alcohol, or lube and continues to beep when pregnancy is detected?
Preg tone®
What are TWO reasons that the A mode U/S is beneficial?
- rapid
- inexpensive
What animal is the A mode U/S most commonly used in?
swine
What U/S produced a 2D image using a 3.5-5 MHz probe that is done transrectal or transabdominal?
B mode
What are the TWO structures that doppler is useful for observing>
• Middle uterine artery
• Umbilical arteries
What is the length of estrous in the sheep?
17 days
What is the gestation length in the sheep?
147 days
What maintains the early pregnancy in the sheep?
CL
What maintains the later pregnancy in the sheep? What are the days that this structure takes over?
Placental progesterone, Day 55-75
What is the placentation in the sheep/goat?
Cotyledonary, epitheliochorial
What is the maternal recognition of pregnancy hormone in sheep/goat?
Interferon Tau
What is the length of estrous in the goat?
21 days
What is the length of gestation in the goat?
150 days
When in the pregnancy can you use the transrectal U/S to diagnose pregnancy?
day 20-40
When in the small ruminant does the uterus fall over the pelvic brim?
day 50-60
T/F The transrectal could give you a false negative later in gestation
True
What anatomical structure can you identify to help you find the uterus?
bladder
What diagnostic can be used the second half of gestation that could be beneficial?
doppler
How far along does the small ruminant need to be to use transabdominal U/S for pregnancy dx?
> 30 days gestation
When can you transabdominal to assess the number of fetuses present?
day 45-60
T/F You need to clip the fiber/wool for the transabdominal U/S
True
How do you ID where the uterus will be for the transabdominal U/S?
Aim toward opposite hip→ identify bladder→ uterus
cranial to bladder (early pregnancy
C shaped placentomes point (concave) (away from/toward) fetus
toward
What can give you a false positive on U/S? (2)
• Hydrometra
• Bladder
What can give you a false negative on U/S? (1)
too early (be sure to recheck)
fetal aging?
fyi i think
How far along does the dam need to be to be able to fetal age?
55-75 days gestation
How do you fetal age?
Visualizing the genital tubercle
Which genital tubercle do you look for first?
male ( migrate cranially just caudal to the umbilicus- penis and prepuce)
When are the fetal skeleton visiable on RADS?
Day 58 post-breeding
When are the fetal skeletons radiopaque?
Day 65
When is the best time to use RADS to avoid false negatives?
> 90 days
T/F If u/s not available.. you can use radiographs to confirm no other fetus present for dystocia
True
What are THREE ways that a teaser ram/buck is used/made?
• Lack or presence of marker
• Epididymized/Vasectomized
• Translocation of penis
What is the #1 signs that the small ruminant has failed to return to estrus?
standing to be mounted
T/F Goats show more signs of heat than sheep
True
What are some other signs that the small ruminant has failed to return to estrus? (3)
• Flagging of tail
• Vocalization
• Respond to buck rag/seek out male
When is Abdominal Ballottement more accurate for diagnosing a pregnancy?
later in pregnancy
Where do you preform Abdominal Ballottement at?
Lower right quadrant of abdomen
What can cause a false positive with ballottement?
Ventral sac of the rumen
What are TWO things that can cause false negatives?
• Fetus not large enough to be balloted
• Inexperience
What is the best use of progesterone in small ruminants?
used to determine that the animal is NOT pregnant
If the P4 is below what range later in the cycle of the goat/ewe dictates that the animal is NOT pregnant?
If the ewe is
18-19 days
If the doe is
19-21 days
T/F If P4 is >1-2 ng/ml indicate functional CL tissue
True
What are the samples you need to be able to test for P4?
milk or serum (serum us more accurate)
What TWO instances can cause false positives?
• Pseudopregnancy
• Persistent CL
What is the hormonal assay that is produced by the feto-placental unit that is seen in urine serum, milk > 50 days gestation?
Estrone Sulfate
T/F Estrone Sulfate is NOT positive with a pseudopregnancy!
True ( this is bolded)
If there is a positive result with estrone sulfate, what does this indicate?
there is a fetus
What are TWO instances that estrone sulfate can cause a false negative?
• < 50 days gestation
• Hemolyzed sample (if serum)
What is the hormone that is produced by binucleated cells of the placenta and can be test > 30 days post breeding?
Pregnancy Specific Protein B
What can cause a false positive with Pregnancy Specific Protein B?
- Early postpartum (Lactating animals)
What can cause a false negative with Pregnancy Specific Protein B?
< 30 days post breeding sample collection
What is the normal estrous in swine?
21 days
What is the gestation length in swine?
114 days (3 month, 3 weeks, 3 days)
What is maintaining the pregnancy in swine?
Cl dependent entire time
What type of placentation do swine have?
Diffuse villous, epitheliochorial
T/F The maternal recognition of swine is not completely known
True
How many piglets per horn in production need to be present for her to maintain the pregnancy?
2/horn
What are the FOUR ways to diagnose pregnancy in swine?
• Ultrasonography
• Radiographs
• Failure to return to estrus
• Hormone/Protein assays
T/F The A mode U/S is transabdominal and is best if the swine are > 30-35 day gestation due to the high false positive rate
True
What day in the gestation can you ultrasound with the B mde and be fairly successful?
> 21-25 day post mating
When can you use the Transabdominal 3.5 mHz B mode U/S to diagnose pregnancy?
Day 24
What can cause a false postive on B mode U/S in swine?
Mucometra (also pyo)
What can casue a false negative on B mode U/S?
Too early (< 22 days) in
gestation
When can you run a progesterone assay on swine? What range tells you if she not pregnant? Pregnant?
• 16-24 days post breeding
• If not pregnant < 6 ng/ml
• Pregnant→ ~ 30ng/ml
T/F > 6 ng/ml of P4 is needed to maintain pregnancy
True
When can you use estrone sulfate in swine to detect pregnancy? What samples can you use?
• Days 24-28 post breeding
• Blood or feces
What is the normal estrous cycle length in the camelid?
induced ovulators
Llama: 19-20 days
Alpaca: 9-17 days
What is the gestation length of alpaca and llama?
Llama: 335-360 days
Alpaca: 327-346 days
What maintains the pregnancy of alpaca and llamas?
CL dependent entire pregnancy
What is the placentation in camelids?
Diffuse, microcotyledonary,
epitheliochorial
T/F We dont know what the maternal recognition is in camelids bc theyre weird :/
True
If a camelid has twins, what happens?
death
What does the term "spitting off the male" mean in regards to camelids?
she is not receptive to the male
What days can you test spitting off in camelids?
Test at day 7 and 14 post breeding
How much progesterone needs to be present for her to spit at the male?
> 2 ng/mL
What does the camelid do if she is receptive?
Cush position
When is the earliest you can do a transrectal U/S?
14 days