Microm 402 UW

Mannitol salt agar

Organisms that can ferment mannitol and also grow on high salt concentrations .

Mannitol salt agar positive result and test for what bacteria?

pink to yellow. Staphylococcus aerus

mannitol ferment tube

organisms that can ferment mannitol

mannitol ferment tube positive result and test for what bacteria?

pink to yellow. Staphylococcus.

glucose shake tube

find if the organism is strict aerobe, strict anaerobe or facultative anaerobe.

glucose shake tube positive result and test for what bacteria?

strict aerobe grow on surface like Micrococcus

strict anaerobe grow 5-10 ml below surface

facultative anaerobe grows throughout like staphylococcus

bromecresol purple slant + 1% glucose+ TSY

Find if organisms are strict aerobe, anaerobe or facutative anaerobe.

bromecresol purple slant + 1% glucose+ TSY positive result and test for what bacteria?

Like shake tube but also shows acid from glucose by changing color from purple to yellow.

novobiocin positive test means what bacteria

S.epidermidis or S.aerus

catalase test

formation of O2 due to presence of catalase enzyme

catalase negative bacteria

Clostridium, Leuconostoc, Lactobaccillus,

bergey's bacillus #1

glucose fermentation

bergey's bacillus #1 positive result and test for what bacteria?

purple to yellow no gas because gas is absorbed in the medium.

Bacillus

bergey's bacillus #2 positive result and test for what bacteria?

bubbles along the stab inoculation.

Bacillus

Acetoin test

Glucose fermentation

Acetoin test positive result and test for what bacteria?

pink colour formation on addition of Voges-Proskauer reagents A and. Bacillus

Methyl red test

acid from glucose fermentation

Methyl red test positive result and test for what bacteria?

red color on addition of methyl red. Bacillus

Yellow is negative

Milk agar

casein hydrolysis

Milk agar positive result and test for what bacteria?

Clear zone formation. Bacillus. Pseudomonas

Starch agar positive result and test for what bacteria?

zone of clearing on addition of Gram's iodide. Bacillus

starch+ glucose agar

catabolite repression by glucose

ethanol chalk agar

oxidation of ethanol to acetic acid

ethanol chalk agar positive result and test for what bacteria?

formation of clear zone. Acetobacter and Gluconobacter

acetate-yeast extract

ability to oxidize acetate

acetate-yeast extract positive result and test for what bacteria?

Growth. Acetobacter

glycerol-yeast extract broth

ability to form cellulose

glycerol-yeast extract broth positive result and test for what bacteria?

cellulose insoluble pellicle on surface. Acetobacter

Oxidase test

Cytochrome C

Oxidase negative bacteria

Acinetobacter, Enterobactericeae, Leuconostoc, lactobacillus, clostridium

King's B agar

diffusible pigement production

King's B agar positive test and bacteria

yellow, diffused pigments sometimes florescence. Pseudomonads

Gelatin deep positive and bacteria

liquefaction of the medium. Psedomonads

EMB agar plate

lactose fermentation (coliforms)

EMB agar plate positive test and bacteria

black/ purplish colonies, with/without eosin green sheen

Indole test

indole formation from tryptophan

Indole test positive test and bacteria

a red layer formation by addition of Kovac's reagent. E.coli

Simmon's Citrate slant

utilization of citrate as sole carbon source

Simmon's Citrate slant positive test and bacteria

Growth with or without green to blue color change. E.coli

orinithin decarboxylase

ability to decarboxylate orinithine in anaerobic condition.

orinithin decarboxylase positive test and bacteria

red-violet color due to orinithine to putracine formation. E.coli

motility medium

haze of growth which may be pink or red due to reduction of dye. E.coli

Volutin Stain

poly-phosphate granules

Volutin Stain positive test and bacteria

spherical reddish purple bodies within blue cell on addition of methylene blue.

PHB stain

poly-B-hydoxybutyrate . blue/gray/black within red/pink cell on addition.

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