Microbiology Lab Final - UTA

What biosafety level is considered 'High Risk'?

BSL-4

T/F: Media can be considered complex or undefined.

True

The autoclave has three main phases:

Purge, Sterilization, Exhaust

T/F: Bacteria can be in three relationships: pathogenic, communalistic, mutualistic

True

Active ingredients can either kill microbes ______ or inhibit growth _________.

Microbiocidal, Microbiostatic

T/F: (Control CFU-Treatment CFU)/Control CFU *100%

True

T/F: When a single bacterial cell is placed onto agar media, it will start to undergo binary fission.

True

Which one of the following is not an example of form (morphology) of a bacterial colony?

Flat

T/F: CFU stands for colony forming unit

True

What is the formal for total magnification?

Objective * Ocular

T/F: Stains are basic if negatively charged and acidic if positive.

False

T/F: This is a gram positive

False

Capsule stain uses what stain?

India Ink, Crystal Violet

T/F: Metabolically active cells are also referred to as vegetative cells.

TRUE

Microorganisms that are ____ rely heavily on the environment for 'ready made molecules'

Fastidious

T/F: Undefined media contains ingredients that are normally derived from extracts of plants, fungi or animal tissue.

True

___ is intended for selection and differentiation of Staphylococcus.

Mannitol Salt Agar (MSA)

T/F: Halophilic are microorganisms capable of growth with or without salinity.

FALSE

T/F: Blood agar has three patterns: Beta, Alpha, Omega

FALSE

T/F: Mitis Salivarius is intended for the isolation as staphlococcus.

FALSE

Bile Esculin is used for isolation of enterococci and group __ streptococci

D

MacConkey agar is intended for the isolation of ____ bacilli

Gram-negative

T/F: Eosin Methylene Blue agar is intended for isolation of gram-positive bacteria.

FALSE

T/F: Thiosulfate Citrate Bile Salt is selective for staphlococcus

FALSE

Biochemical tests performed in lab 6 can detect all the following except:

Growth rates

Select all the organisms that are capable of growth on MSA medium.

Staphylococcus aureus, Staphylococcus epidermidis

T/F: Safranin can be used as a simple stain.

TRUE

The difference between gram-positive and gram-negative cells that causes them to stain differently after Gram staining?

Gram-positive cells have thicker peptidoglycan layers in their cell walls

Which stains/reagents are required for the endospore staining procedure?

De-ionized water, Malachite green, Safranin

T/F: According to the capsule staining exercise, Klebsiella pneumoniae was able to produce a capsule while Escherichia coli

TRUE

T/F: Immersion oil is required for 4X, 10X, 40X, and 100X objective lenses.

FALSE

Starch, casein, gelatin, and urea hydrolysis are all performed by microbes using which following molecule(s)?

water , enzymes

All of the following tests involve solid agar media except:

SIM Test

T/F: A microbe that can ferment dextrose, reduce sulfur, and produces gas would show black participate and splitting agar

TRUE

Which of the following is evidence of catalase production?

production of O2 gas bubbles

T/F: Both of the MR and VP tests use a broth containing peptone, glucose, and phosphate buffer.

TRUE

Which organisms would grow best on TCBS agar? Select all that apply.

Vibrio harveyi , Vibrio cholerae

In which phase of an autoclave cycle does the exhaust valve shut and allow for pressure and steam

Sterilization phase

What is the function of coagulase?

to convert fibrinogen to fibrin

T/F: When focusing a light microscope, it is best to adjust the the fine focusing knob before first before using the course

FALSE

What tests can you additionally perform to confirm the presence of a fecal coliform? Select all that apply.

Indole test , Citrate utilization test , MR-VP test