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Photosynthesis equation
6H₂O + 6CO₂ → C₆H₁₂O₆ + 6O₂ in the presence of light energy;
Reaction type of photosynthesis
Redox reaction where CO₂ is reduced to glucose and H₂O is oxidized to O₂ (oxygen comes from water, not CO₂);
Origin of photosynthesis biologically
Likely began in prokaryotes with chloroplasts as the main photosynthetic structure;
Autotroph definition
Organisms that make their own organic compounds from inorganic sources using energy (usually light);
Heterotrophs and consumers
Depend on other organisms for food (ex: animals, fungi, decomposers like bacteria);
Photoautotroph examples
Green plants, algae, euglena, cyanobacteria, purple sulfur bacteria;
Main photosynthetic organ
The leaf;
Leaf structure
Composed of upper/lower epidermis, stomata, mesophyll, and veins;
Function of stomata
Openings (mainly on the underside) that release oxygen and take in CO₂;
Chloroplast location in leaf
Concentrated near upper surface in the palisade mesophyll to maximize light absorption;
Spongy mesophyll
Loosely arranged cells with air spaces that allow gas exchange;
Chloroplast count per leaf cell
About 30–40;
Relationship between photosynthesis and respiration
Complementary processes—photosynthesis produces glucose and O₂ used by respiration, while respiration produces CO₂ and H₂O used by photosynthesis;
Photosynthesis stages
Light reactions (dependent on light) and Dark reactions (Calvin Cycle, light-independent);
Light reactions location
Thylakoid membranes of chloroplasts;
Dark reactions location
Stroma of chloroplasts;
Light reaction inputs
Water, light energy, ADP, NADP⁺;
Light reaction outputs
Oxygen, ATP, NADPH;
Purpose of light reaction
Convert light energy to chemical energy (ATP and NADPH) and release O₂;
Dark reaction inputs
CO₂, ATP, NADPH;
Dark reaction outputs
adds CO2 and produces sugar; G3P (glyceraldehyde-3-phosphate) → converted to glucose and sucrose;
Electromagnetic spectrum and light
Visible light is a small part (380–740 nm) used in photosynthesis;
Light behavior
Dual nature—acts as both a particle (photon) and a wave (measured in nm);
Color absorption
Red, blue, and violet light absorbed best;
Green light
Reflected, making plants appear green;
Green plant light interest
Focus on light quality, quantity, and duration;
Quality of light definition
Describes the wavelength or color composition of light affecting photosynthesis;
Absorption spectra
Graph showing which wavelengths of light pigments absorb best (chlorophyll absorbs blue and red light strongly);
Action spectra
Graph showing photosynthetic rate versus wavelength—matches the absorption spectrum, highest in blue and red;
Spectrophotometer use
Measures absorbance of light at different wavelengths to determine pigment activity;
Engelmann’s experiment
Used algae and bacteria to show that photosynthesis occurs most under blue and red light (where oxygen production is greatest);
Green shade effect
Plants grown in green light grow poorly because green light is reflected, not absorbed;
Quantity of light
Amount of light energy available for photosynthesis, measured in foot-candles or lux;
Duration of light (Photoperiod)
Length of exposure to light; affects flowering and growth cycles (long-day, short-day, and day-neutral plants);
Chlorophyll structure
Pigment molecule with magnesium at the center of a porphyrin ring; Unique because magnesium electrons become excited and move to higher energy states;
Types of chlorophyll
Chlorophyll a (main pigment), chlorophyll b and others as accessory pigments;
Light reaction overview
Photosystems (PSII and PSI) are light-harvesting complexes that capture light and convert it into chemical energy;
PSII
Absorbs light at 680 nm; splits water molecules (photolysis) releasing O₂, protons (H⁺), and electrons;
Electron pathway
Electrons from PSII travel down an electron transport chain (ETC), losing energy that is used to pump protons and generate ATP by chemiosmosis and ATP synthase;
PSI
Absorbs light at 700 nm; re-energizes electrons that reduce NADP⁺ → NADPH using enzyme NADP⁺ reductase;
End products of light reaction
O₂ (from water), ATP (from chemiosmosis), NADPH (electron carrier);
Calvin Cycle (dark reaction) function
Uses ATP and NADPH to convert CO₂ into sugars;
Calvin Cycle phase 1 (Carbon fixation)
CO₂ enters through stomata and binds to RuBP (5-carbon sugar) via enzyme Rubisco, forming a 6C intermediate that splits into two 3C molecules (3-PGA);
Phase 2 (Reduction)
3-PGA converted to G3P using ATP and NADPH; some G3P leaves to make glucose, others stay in cycle;
Phase 3 (Regeneration)
Remaining G3P used to regenerate RuBP using ATP, allowing cycle to continue;
Each full Calvin cycle
Fixes 3 CO₂ molecules and produces one G3P;
C3 plants
Named for 3C compound (3-PGA) formed first; common agricultural plants like rice, wheat, soybeans, and vegetables;
C3 plant disadvantage
In hot, dry conditions they close stomata to conserve water, reducing CO₂ intake and causing photorespiration;
Photorespiration
Occurs when Rubisco binds O₂ instead of CO₂, wasting energy and reducing sugar production (up to 50% carbon loss);
C4 plants
Include corn, sugarcane, and grasses; Adaptation
C4 plant mechanism
Stomata partially close in heat, CO₂ stored in 4C compound, later released to Calvin Cycle;
C4 plant productivity
30–40% higher than C3 plants like rice;
CAM plants
Include pineapple, cacti, aloe, and other succulents;
CAM plant adaptation
Open stomata at night to take in CO₂ and close during day to prevent water loss; CO₂ stored as organic acids at night, used in Calvin Cycle during the day;
CAM plant advantage
Efficient water conservation in arid environments;
Sugar use
Sugars from photosynthesis converted into cellulose for plant structure;
Cellulose
Most abundant carbohydrate on Earth;
Global cellulose production
About 150 billion metric tons produced annually.