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willow spring high school
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three questions when examining blood stain
is stain actually blood, what species, what type of blood if human
role of RBC
most abundant, antigens, produced in bone marrow, contains hemoglobin
role of WBC
work with immune system, contains nucleus DNA
tests to determine if stain is blood
kastle-meyer (PINK), hemastix (GREEN), luminol
main chemical in kastle-meyer test
phenophthalein PINK
test used to determine if human or not
preciptin test (antibodies)
importance of using luminol
very sensitive, indicates old blood or tiny drops, doesn't interfere with DNA
where are antibodies found
proteins formed by WBCs carried in plasma based upon what blood type you are (your antigen)
your blood type
your antigen
Type A blood contains these antigens and antibodies
A antigens, anti-B antibodies
type AB blood contains these antigens and antibodies
A+B antigens, no antibodies
clumping
agglutination
mom- A dad- O
A or O
O positive
most common
AB negative
least common
angle of impact formula
sin(angle) = width/length
satelites
little dots away from splatter
low velocity has this diameter
3mm or larger
medium velocity hsa this diameter
1-3 mm
high velocity has this diameter
1 or less mm
cast-off spatter
blood swinging off a weapon
expiratory blood spatter
from lungs/sinus
blood spatter on smooth surface
uniform, circular, small, smooth edges
blood spatter on medium rough surface
crenelated edges with spines
blood spatter on rough surface
parent drop with satelites
serology
study of blood
where antigens are found
protein markers that determine blood type in RBCs