bio microscopes and cell theory

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33 Terms

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Light Microscope

Uses visible light for illumination.

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Electron Microscope

Uses a beam of electrons for illumination.

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Magnification of Light Microscope

Up to ~1,000x.

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Magnification of Electron Microscope

Up to ~2,000,000x.

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Resolution of Light Microscope

~0.25-0.3 µm.

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Resolution of Electron Microscope

~0.001 µm.

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Specimen Type for Light Microscope

Can observe live or dead specimens.

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Specimen Type for Electron Microscope

Can only observe dead or dried specimens.

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Image Output of Light Microscope

Produces colored images.

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Image Output of Electron Microscope

Produces black and white images (can be colorized digitally).

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Lens Material of Light Microscope

Made of glass.

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Lens Material of Electron Microscope

Uses electromagnetic lenses.

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View Format of Light Microscope

2D (some stereo microscopes offer 3D).

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View Format of Electron Microscope

2D or 3D depending on type (TEM or SEM).

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Preparation Time for Light Microscope

Minutes to hours.

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Preparation Time for Electron Microscope

Several days.

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Portability of Light Microscope

Compact and movable.

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Portability of Electron Microscope

Large, stationary equipment.

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Cost of Light Microscope

Affordable for schools and hobbyists.

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Cost of Electron Microscope

Expensive; used in advanced labs.

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Applications of Light Microscope

Used in education, basic biology, and live cell study.

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Applications of Electron Microscope

Used in nanotech, virology, and materials science.

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Field of View (FOV)

The diameter of the visible area under the microscope.

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Relationship between Magnification and FOV

As magnification increases, the field of view decreases.

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Cell Theory - First Tenet

All living organisms are composed of one or more cells.

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Cell Theory - Second Tenet

The cell is the basic unit of structure and function in living things.

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Cell Theory - Third Tenet

All cells arise from pre-existing cells.

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Robert Hooke

Coined the term 'cell' after observing cork under a microscope.

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Matthias Schleiden

Proposed that all plants are made of cells.

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Theodor Schwann

Concluded that all living things are composed of cells.

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Rudolf Virchow

Added the tenet that all cells arise from pre-existing cells.

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Conversion of Millimetre to Micrometre

1 mm = 1000 µm.

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Example of Conversion

15 mm = 15 × 1000 µm = 15000 µm.