A2.2.2 - Microscopy Skills
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10 Terms
What are temporary mounts used for?
to quickly observe dynamic processes of living or fresh specimens (like cell movement or division) without complex preparation
What do stains do?
enhance the visibility of cellular structures by adding contrast
What are the two different kinds of stains and their differences?
methylene blue: stains nuclei
iodine: stains starch granules
What are the three steps to focus a light microscope?
position slide
course focus - location
fine focus - clarity
What is an Eyepiece Graticule?
a scale etched onto the eyepiece lens that allows measurements in eyepiece units (EPU)
calibration
the process of determining the actual length represented by one EPU at a specific magnification
what is a micrometer?
a slide with a known scale
Why is calibration necessary when using EPUs?
because the size of one EPU varies with magnification, and calibration determines the actual length represented by one EPU
What are the three essential guidelines for biological drawings?
clean, continuous lines (no shading) in pencil
label structures with straight lines in pen
include mag. or scale bar
why are quantitative observations important?
they provide precise data that can be compared and analyzed
Note 
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