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Cataplerotic reactions
Deplete the components of the cycle
Products are consumed in other pathways
Anaplerotic reactions
Replenish the components of the cycle
Products from other pathways are converted to substrates of the cycle
Step 1:
Oxaloacetate → Citrate
This is a very exergonic reaction due to the hydrolysis of the acetyl-CoA thioester
Step 2:
Citrate → Isocitrate
Step 3:
Isocitrate → α-Ketoglutarate
Step 4:
a-ketoglutarate → succinyl-CoA
Enzyme: α-Ketoglutarate dehydrogenase
Step 5:
Succinyl-CoA → Succinate
This is the only substrate-level phosphorylation reaction of the cycle
(right to left in pic)
Step 6:
Succinate → Fumarate
(right to left in pic)
Step 7:
Fumarate → Malate
Step 8:
Malate → Oxaloacetate