OCHEM LAB Safety

What is rule number 1 of the General Safety Rules?

The goggles provided by UT must be worn at all times

2. What should you wear to be properly dressed in the lab? (a. b. c.)

a. close toed shoes

b. at least a short sleeve t-shirt

c. pants

What is rule number 3 of the general safety rules?

No eating or drinking in the lab

What is rule number 4 of the general safety rules?

Lab coats provided by UT must be properly worn at all times

What is rule number 5 of the general safety rules?

No horseplay in the lab

What is rule number 6 of the general safety rules?

Dispose of chemicals properly

What is rule number 7 of the general safety rules?

Know where all the safety equipment is located

What should you do if an accident occurs? (no. 8)

Notify your TA

Rule 9 of general safety rules

follow the procedure of the lab and do not attempt any experiments that have not been authorized

Rule 10 of general safety rules

Clean your area after you have finished the lab

Rule 11 of general safety rules

Wash your glassware properly and dispose of broken glassware in the proper container

Rule 12 of general safety rules

Do not run in the lab, walk calmly

Rule 13 of general safety rules

Give people room to move around the lab and do not crowd around other people. Especially if they are carrying lab equipment or chemicals.

Rule 14 of general safety rules

Dedicate a pen strictly for lab to limit exposure of chemicals outside of lab. Never put this pen in your mouth or use if wearing gloves.

Rule 15 of general safety rules

Treat everyone in the lab with respect and courtesy

Rule 16 of general safety rules

Avoid contact of the chemicals on your skin. If you do come in contact, wash the area properly.

SOP C: Disposing of waste - how to dispose of solid chemical waste?

1. Solid waste is considered any solid chemicals that you no longer need. Doesn't include gloves, paper towels, or weigh boats.

2. Dispose of solid waste in the solid waste container

SOP C: Disposing of waste - how to dispose of trash?

1. Trash is considered things like weigh boats, paper towels, and gloves.

2. Dispose of in trash cans located throughout lab.

SOP C: Disposing of waste - how to dispose of broken glass?

1. Dispose of broken glass in the broken glass bins throughout the lab.

2. Be careful when handling broken class and to not cut yourself.

SOP C: Disposing of waste - how to dispose of liquid chemical waste?

1. Dispose of all liquid waste in the proper container.

2. Be sure to lift the lid before emptying or pouring the waste into the container.

3. If the container is full, inform the TA so a new one can be obtained

4. Ensure that all acids or bases are neutralized before they are disposed into the aqueous waste container.

SOP C: Disposing of waste - how to dispose of sharps waste (needles, syringes, razors)?

1. Dispose all sharps waste into waste container

2. Never throw it into the trash. All needles will be accounted for and disposed of properly. If a needle is missing, no one will be allowed to eave the lab until it is found.

(SOP D: Wearing gloves) What is the most common way to be exposed to chemicals?

On the hands

SOP D: How do you wear gloves properly?

1. Find gloves that fit properly and are not too big or small

2. Wear gloves only when handling chemicals and do not touch yourself when you are wearing gloves.

3. Inspect gloves while you wear them for rips or tears. If there are rips or tears dispose of them properly in the trash can and get new ones. If they are exposed to chemicals dispose of the gloves properly and obtain new ones.

4. After you are done handling chemicals, take the gloves off because you no longer need them.

5. Remove and dispose of gloves properly before leaving the lab.

SOP B: What should you do when working with organic chemicals?

1. Be careful not to be exposed when working with organic chemicals because many are toxic

2. Wear gloves when handling organic chemicals and always inspect gloves. If the gloves are exposed, dispose of the gloves and obtain new ones while you are still working with the chemicals until you no longer need the gloves.

3. When handling a liquid organic chemical, transfer it inside the hood and then quickly move it to your own hood to avoid inhaling the vapors.

4. When working with solids, carefully weigh the appropriate amounts on the weigh boat and never put any chemical back into the original container.

5. Have MSDS on hand for all the chemicals you will be using so that if you need any information you can get it quickly.

(SOP A: Glassware): What should you always do before using glassware?

2. Inspect it for any cracks

(SOP A: Glassware): Why is it important to use glassware properly?

1. To ensure you avoid any spills or breakages.

(SOP A: Glassware): What should you do at the end of lab and what should you be careful of?

Clean glassware thoroughly at the end of lab and be careful cleaning because most cuts occur when students are cleaning glassware.

(SOP A: Glassware): (4.) You must always clamp your glassware properly when assembling a glassware set up. What happens if you use too many clamps? When should you use Keck clamps?

Using too many clamps makes the setup inflexible while using too few clamps may result in the apparatus falling apart.

Use Keck clamps when you have two pieces of glassware connected by a glass joint.

(SOP A: Glassware): If instructed to do so, what should you do to the joints of the glass?

Grease them

(SOP A: Glassware): What should you ensure occurs when using a thermometer adapter.

That it is inserted properly

(SOP A: Glassware): What should you ensure occurs when using a rubber-septa.

That it is inserted properly

(SOP A: Glassware): Describe what to do if you break any glassware?

Let your TA know immediately and clean up mess if it is small enough for you to handle. Place broken glass in proper container and ensure you don't cut yourself.

(SOP A: Glassware): When obtaining a chemical, what should you keep in mind?

Use the correct size container when getting a chemical. Ex. If you need 50 mL get a container larger than 50 mL to avoid spills.

SOP I (heating reactions):

1. Why is it important to handle organic compounds carefully?

2. What should you make sure to do when heating a reaction?

3. What should you NEVER do when heating a reaction?

4. What phrases should you pay attention to when working with exothermic reactions such as acid and base? Why?

1. Most organic compounds can catch fire, especially solvents.

2. Ensure it is not a closed system, because this will build up pressure quickly.

3. Leave it unattended.

4. "Add dropwise" or "slowly add" because exothermic thermic reactions can get out of control quickly.

EOP: Exposure to chemicals - What should you do if your eyes are exposed to chemicals?

Go to the eyewash station and rinse your eyes for a minimum of 15 minutes.

EOP: Exposure to chemicals - What should you do if your face is exposed to chemicals?

If you are exposed to a chemical that can be rinsed out with water, go to the eyewash station. Rinse for at least 15 minutes. If not, refer to the MSDS sheet.

EOP: Exposure to chemicals - What should you do if you spill onto your coat?

Remove the coat immediately and get a new one

EOP: Exposure to chemicals - What should you do if a large area of your body is exposed to a chemical?

Go to the shower and rinse for 15 minutes. If it is a small area, it may be required to rinse at the sink.

EOP: Exposure to chemicals - What should you do if you have been overcome by the vapors of a chemical? How do you prevent exposure to vapors from organic compounds?

Let the TA know ASAP and go get some fresh air. Also follow the instructions on the MSDS sheet.

Avoid exposure by transferring liquid chemicals inside the hood and quickly transfer them into your own hood.

EOP: Fires - What is the most important thing to remember when there's a fire?

Stay calm

EOP: Fires - What is the first thing you should do when there is a fire? Can you use the fire extinguisher?

Shout "fire" loudly and if it is small use the fire extinguisher.

EOP: Fires - What should you do if your clothes catch on fire?

Smother the flames quickly with a fire blanket

EOP: Fires - What should you do if the fire is too big?

Evacuate and pull the fire alarm. Follow evacuation protocols.

EOP: Spills - What should you do first if there is a spill?

Notify the TA immediately and together you will evaluate the spill and whether you can clean it up or if the stockroom should.

EOP: Spills - What should you if you are in charge of cleaning up a spill?

If no spill kit is required, clean up spill thoroughly and wear gloves while doing so. Be careful if broken glass is involved.

EOP: Spills - What should you do if there's waste?

Dispose of the waste in the proper container.

EOP: Evacuations - What should you do in order if you are to evacuate?

1. First, turn off all equipment

2. Second, follow classmates and TA out of the lab to the assigned evacuation point.

3. Stay as a group and do not leave unless instructed to by the TA or the lab instructor.

4. Fourth, only re-enter the lab once you have been instructed to by the TA or the lab instructor.

EOP: Accidents - What can accidents result in?

Injuries, fires, spills, burns, or damaged equipment

EOP: Accidents - What should you do if there's an accident?

Notify your TA immediately

EOP: Accidents - What will happen if you are hurt?

Injury will be assessed and determined if it can be taken care of my the lab or if needed medical assistance. A accident form will also be filled out

EOP: Accidents - What will always be an option if you are hurt in the lab?

Medical assistance

EOP: Electrical - Why are electrical hazards dangerous? Name a source.

Electrical hazards can result in death or serious injury. There are many sources such as faulty wiring or faulty equipment.

EOP: Electrical - How can you avoid electrical hazards? Where should you plug in all equipment if it is near a water source?

By using electrical equipment properly and not for what they were not designed to do.

Checking the power cables for damage before turning the equipment on

Plug into a Ground-Fault Circuit Interrupter

EOP: Electrical - What should you do if you see someone who has been electrocuted?

Do not touch them to avoid being electrocuted yourself - most important thing!!!

Call 911 immediately and if possible turn off equipment or trip the breaker (do not attempt if not safe)

Describe the fire hazard exposure, prevention, and what to do if exposed for Toluene.

Hazard/symptoms: severe fire hazard, vapors, and gases may ignite if near ignition sources

Prevention: No sparks, no flames, no contact with hot surfaces

First aid/fire: Regular dry chemical, carbon dioxide, water for cooling, regular foam

Describe the inhalation hazard exposure, prevention, and what to do if exposed for Toluene.

Hazard/Symptoms: fatigue, weakness, confusion, headache, drowsiness, nausea

Prevention: ventilation and local exhaust

First aid/fire: Remove from exposure immediately; get medical attention

Describe the skin hazard exposure, prevention, and what to do if exposed for Toluene

Hazard/Symptoms: irritation and drying

Prevention: protective gloves and clothing

First aid/fire: Remove contaminated clothes and jewelry and wash skin thoroughly

Describe the eye hazard exposure, prevention, and what to do if exposed for Toluene

Hazard/Symptoms: irritation and corneal burns

Prevention: safety goggles

First aid/fire: wash eyes with water for several minutes, remove contact lenses if possible, seek medical attention immediately

Describe the ingestion hazard exposure, prevention, and what to do if exposed for Toluene

Hazard/Symptoms: abdominal spasms, systemic effects given in inhalation, coughing, and gagging.

Prevention: Do not eat or drink in laboratory

First aid/fire: Contact physician immediately and keep head lower than hips if vomiting

Describe the carcinogenicity and mutagenicity of toluene.

Toluene is not a known carcinogen but is a possible mutagen

Describe the carcinogenicity and mutagenicity of acetone.

Acetone is not a known carcinogen but is a possible mutagen

Describe the ingestion hazard exposure, prevention, and what to do if exposed for Acetone

Hazard/Symptoms: Fruity odor of breath and mucous membrane. Gastroenteric irritation, flushed cheeks, shallow respiration, latent period followed by restlessness, diarrhea, vomiting, and nausea.

Prevention: Do not eat or drink in laboratory

First aid/fire: Contact physician immediately and keep head lower than hips if vomiting

Describe the eye hazard exposure, prevention, and what to do if exposed for Acetone

Hazard/Symptoms: Irritation and corneal epithelial, conjunctival, stinging sensation, an damage to eyes

Prevention: Safety goggles

First aid/fire: wash eyes with water for several minutes, remove contact lenses if possible, seek medical attention immediately

Describe the skin hazard exposure, prevention, and what to do if exposed for Acetone

Hazard/Symptoms: Irritation, cellular damage to outer layer of epithelium with edema, hyperemia, small amounts may be observed through in tact skin

Prevention: Gloves and protective clothing

First aid/fire: Remove contaminated jewelry and clothing. Wash skin thoroughly with water.

Describe the inhalation hazard exposure, prevention, and what to do if exposed for Acetone

Hazard/Symptoms: Irritation, dryness of mouth and throat, anorexia, restlessness, vomiting, slow irregular respiration, weak pulse, liver and kidney damage, central nervous system depression with weakness of legs, headache, nausea, and a general feeling of malaise.

Prevention: Ventilation and local exhaust

First aid/fire: Remove from exposure immediately and get medical attention

Describe the fire hazard exposure, prevention, and what to do if exposed for Acetone

Hazard/symptoms: severe fire hazard, vapors, and gases may ignite if near ignition sources

Prevention: No sparks, no flames, no contact with hot surfaces

First aid/fire: Alcohol resistant foam, CO2, regular dry chemical, water

Describe the fire hazard exposure, prevention, and what to do if exposed for Cyclohexane

Hazard/symptoms: severe fire hazard, vapors, and gases may ignite if near ignition sources, moderate explosion hazard

Prevention: No sparks, no flames, no contact with hot surfaces

First aid/fire: Regular dry chemical, CO2, water for cooling, regular foam

Describe the inhalation hazard exposure, prevention, and what to do if exposed for Cyclohexane

Hazard/symptoms: irritation, Central nervous system depression with headache, dizziness, dullness, nausea, vomiting, degenerative changes in the heart, lungs, liver, kidneys, brain

Prevention: Ventilation, local exhaust

First aid/fire: Remove from exposure and seek medical attention

Describe the skin hazard exposure, prevention, and what to do if exposed for Cyclohexane

Hazard/symptoms: Irritation, redness, dryness

Prevention: Protective gloves and clothing

First aid/fire: Remove contaminated jewelry and clothes and wash skin thoroughly with water

Describe the eye hazard exposure, prevention, and what to do if exposed for Cyclohexane

Hazard/symptoms: Irritation, redness, conjunctivitis, tearing

Prevention: Safety goggles

First aid/fire: Thoroughly was eyes for several minutes and remove contact lenses if possible. Seek medical attention immediately.

Describe the ingestion hazard exposure, prevention, and what to do if exposed for Cyclohexane

Hazard/symptoms: CNS depression; aspiration may result in pneumonitis

Prevention: Do not eat or drink in the lab

First aid/fire: Contact physician immediately. Give water or milk; if vomiting keep head lower than hips.

Describe the carcinogenicity and the mutagenicity of cyclohexane.

Not a known carcinogen and possible mutagen

Describe the carcinogenicity and the mutagenicity of ethylbenzene.

Possible carcinogen and possible mutagen

Describe the fire hazard exposure, prevention, and what to do if exposed for ethylbenzene.

Hazard/symptoms: Severe fire hazard, vapors and gases may ignite if near ignition source

Prevention: No flames, no sparks, no contact with hot surfaces

First aid/fire: Regular dry chemical, CO2, water for cooling, regular foam

Describe the inhalation hazard exposure, prevention, and what to do if exposed for ethylbenzene.

Hazard/symptoms: severe irritation, cough, fatigue, headache, dizziness

Prevention: Ventilation and local exhaust

First aid/fire: Remove from exposure immediately, get medical attention immediately

Describe the skin hazard exposure, prevention, and what to do if exposed for ethylbenzene.

Hazard/symptoms: irritation, inflammation, 1st or second degree burns, absorption through skin may cause systemic toxicity

Prevention: protective gloves and clothing

First aid/fire: Remove from exposure immediately, get medical attention immediatelyRemove contaminated clothes and jewelry and wash skin thoroughly. GET MEDICAL ATTENTION IMMEDIATELY

Describe the ingestion hazard exposure, prevention, and what to do if exposed for ethylbenzene.

Hazard/symptoms: abdominal pains, nausea, vomiting, aspiration with extensive edema, and hemorrhage of lung tissue

Prevention: do not eat or drink in the lab

First aid: contact physician immediately. Drink water or milk. If vomiting keep head lower than hips

Describe the eye hazard exposure, prevention, and what to do if exposed for ethylbenzene.

Hazard/symptoms: irritation and lacrimation

Prevention: safety goggles

First aid: wash eyes with water for several minutes, remove contact lenses if possible, seek medical attention immediately

Describe the ingestion hazard exposure, prevention, and what to do if exposed for heptane.

Hazard/symptoms: abdominal cramps, burning sensation, nausea, vomiting

Prevention: do not eat or drink in the lab

First aid: Rinse mouth. Do not induce vomiting. Rest. Refer for medical attention

Describe the carcinogenicity and mutagenicity of heptane.

Not a know carcinogen and possible mutagen

Describe the eye hazard exposure, prevention, and what to do if exposed for heptane.

Hazard/symptoms: Redness, pain

Prevention: goggles

First aid: wash eyes with water for several minutes, remove contact lenses if possible, seek medical attention immediately

Describe the skin hazard exposure, prevention, and what to do if exposed for heptane.

Hazard/symptoms: Dry skin

Prevention: Protective gloves and clothing

First aid: Remove contaminated clothing/jewelry and thoroughly wash area for a few minutes with water and soap. Wear protective clothing and gloves when administering first aid.

Describe the inhalation hazard exposure, prevention, and what to do if exposed for heptane.

Hazard/symptoms: Dullness and headache

Prevention: Ventilation and local exhaust

First aid: Fresh air, rest, refer for medical attention

Describe the fire hazard exposure, prevention, and what to do if exposed for heptane.

Hazard/symptoms: severe fire hazard

Prevention: No flames, no sparks, no contact with hot surfaces.

First aid: Powder, AFFF, foam, CO2, NO water

Describe the fire hazard exposure, prevention, and what to do if exposed for hexane.

Hazard/symptoms: severe fire hazard, vapor may travel considerable distance to ignition source and flash back

Prevention: No flames, no sparks, no contact with hot surfaces.

First aid: CO2, dry chemical powder, or appropriate foam

Describe the inhalation hazard exposure, prevention, and what to do if exposed for hexane.

Hazard/symptoms: Toxic by inhalation. Vapor is irritating to upper respiratory track. Headache, dizziness, and nausea

Prevention: Ventilation and local exhaust

First Aid/Fire: Remove to fresh air. If breathing is difficult give oxygen. Seek medical advice

Describe the skin hazard exposure, prevention, and what to do if exposed for hexane.

Hazard/symptoms: may cause skin irritation

Prevention: Protective clothing and gloves

First Aid/Fire: Immediately flush skin with soap and copious amounts of water for at least 15 minutes.

Describe the eyes hazard exposure, prevention, and what to do if exposed for hexane.

Hazard/symptoms: may cause eye irritation

Prevention: safety goggles

First Aid/Fire: Immediately flush eyes with copious amounts of water, lifting upper and lower lids occasionally. Seek medical advice.

Describe the ingestion hazard exposure, prevention, and what to do if exposed for hexane.

Hazard/symptoms: toxic by ingestion. Symptoms may include headache, dizziness, and nausea

Prevention: do not eat/drink in lab

First Aid/Fire: if swallowed, wash out mouth with water.

Describe the carcinogenicity and mutagenicity of hexane.

Not a known carcinogen and not a known mutagen.