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gram stain
crystal violet, iodine, acetone/alcohol, and safranin
capsule stain
congo red and manevals modified stain
acid fast stain
carbolfuchsin, acid alcohol, and methylene blue
endospore stain
malachite green and safranin
crystal violet length
1 minute
iodine length
1 minute
acetone/alcohol length
brief 5 secs
safranin length
1 minute
congo red length
air dry
maneval modified stain length
1 minute
carbolfuchsin length
10 minutes
acid alcohol length
water and acid rinse mix
methylene blue length
2 minutes
malachite green length
10 minutes
safranin length for endospore staining
2 minutes