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Transcription
the process of making mRNA from DNA, happens in the nucleus
Translation
the process of making protein from the mRNA, happens in the cytoplasm
tRNA
clover shaped, one end carries the amino acid the other carries the anti
mRNA
messenger RNA, made from DNA template strand
anticodon
complements mRNA codon, three bases long, on tRNA
codon
sequence of three bases in mRNA
rRNA
ribosomal RNA, makes up the ribosome
DNA fingerprinting
The analysis of DNA from samples of body tissues or fluids in order to identify individuals.
RNA polymerase
enzyme that links together the growing chain of RNA nucleotides during transcription using a DNA strand as a template
Exons
expressed sequence of DNA; codes for a protein
Introns
Noncoding segments of nucleic acid that lie between coding sequences.
RNA processing
The modification of mRNA before it leaves the nucleus that is unique to eukaryotes.
5' cap and poly a tail
protect mRNA in cytoplasm and tell ribosome where to begin translation
Ribosomes
translate mRNA into specific amino acid sequences
Gene mutation
change in nitrogen base sequence of DNA
Enzyme that plays a major role in transcription
RNA polymerase
insertions and deletions
are mutations that change all amino acids after the mutation
What is the purpose of transcription?
to create mRNA from DNA
What is the purpose of translation?
to create polypeptides and, eventually, proteins for gene expression
Nonsense mutation
A mutation that changes an amino acid codon to one of the three stop codons, resulting in a shorter and usually nonfunctional protein.
Silent mutation
alters a base but does not change the amino acid and has no effect
Missense mutation
A point mutation in which a codon that specifies an amino acid is mutated into a codon that specifies a different amino acid.
Epigenetics
the study of influences on gene expression that occur without a DNA change; identical twins over their lives
spliceosome
enzymes that serves to splice out the introns of a pre
Gel electrophoresis
The separation of nucleic acids or proteins, on the basis of their size and electrical charge, by measuring their rate of movement through an electrical field in a gel.