biol lab final

RED

restriction enzyme digest

PCR

polymerase chain reaction

SNP

single nucleotide polymorphism

dsDNA

double stranded dna

ssDNA

single stranded DNA

bp

base pair

Abs

absorbence

M

molar

mM

millimolar

m

milli

u

micro

m (prefix)

meter

m (metric unit of length)

meter

L (metric unit of volume)

liter

g (metric unit of mass)

gram

The symbol for microliters is

ul

convert 8.5 milliliters to microliters

8500 ul

Which students can taste PTC as bitter?

a, b

which student has two copies of the T version of TAS2R38 gene?

a

what is the phenotype of student c?

nontaster

what is the genotype of student a

homozygous dominant

nontaster is homozygous recessive

true

little taster is heterozygous

true

super taster is homozygous dominant

true

the mixture of DNA fragments of known sizes is used as a reference (arrow) to determine the size of unknown DNA fragments is called ____ _____

DNA ladder

the smallest DNA fragments in the restriction enzyme digest RED is

c

proteins that cut DNA at specific recognition sequences are called ______ ________

restriction enzymes

the ‘ends’ of these DNA fragments are described as _______ ____

sticky ends

name the lab technique that separates DNA fragments based on size

gel electrophoresis

depressions in the gel (enclosed by boxes) where samples are loaded are called ____

wells

what does PCR stand for

polymerase chain reaction

name this lab equipment that you used to perform PCR

thermal cylinder

during this step of PCR reactions, primers bind to single stranded dna

annealing

during this step of pcr reactions, dna polymerase synthesizes new dna strands

extension

what is the concentration mM of a solution with absorbency of 1.4 A

80mM

which term in the linear regression equation indicates how well the line generated by excel fits the data of the class

r2

how many chromosomes does a diploid human cheek cell have

2

the different versions of the gene, T and t, are called ___

alleles

an organisms observable characteristics, such as PTC taster or nontaster is ______

phenotype

name the scientist who discovered the genetic nature of the PTC receptor protein

fox

the subunits of DNA are called

nucleotides

what is the net charge of DNA

negative

the process of breaking down the plasma membrane and nuclear envelope of cheek cells to extract DNA is called ___

lysis

the dna extracted is in __

A

name this lab equipment that you used to break open cheek cells

heat block

describe the fluidity of cheek cell membranes at 99°c

more fluid

after the cell lysis mixture is centrifuged, the liquid at the top of the tube is called ____

supernatant

name this lab equipment that you used to measure the amount of light absorbed by a solution

spectrophotometer

will a working solution have a greater absorbency than a stock solution?

no. false

name part a of the micropipette

plunger

name part b of the micropipette

eject tip

what micropipette will you use to measure 530 microliters of NaCl solution

p1000

the micropipette plunger is depress to the( first or second )stop to pull up the liquid

first

what is the volume range for the p200 micropipette

20-200ul

what volume is displayed

110ul

what stock solution is needed to make 500ml of 5m working solution

25ml

what volume of water is needed to make the working solution

475ml

convert 750 microliters of water to milliliters

.750ml

the density of water is 1g/ml what is the mass g of 750 ul of water

.75g

the concentrated solution that is diluted to make a working solution is called the ____ solution

stock

blue dye is diluted with water. water is the ____ in this solution

solvent

the curved surface of the liquid in the graduated cylinder is called the ____

meniscus

what is the volume of the liquid

25ml