Antigen Recognition

What do B cells recognize

Protein, polysaccharide, lipid, small chemical

What do T cells recognize

Protein antigen

What cells are APC

dendritic cell, macrophage and B lymphocyte

Where are dendritic cells found

Epithelia, peripheral lymphoid organs

Where can classical DC be found

Tissue and lymphoid organ

Where can plasmacytoid DC be found

Blood and tissue

Which TLR is expressed by classical DC

4,5,8

Which TLR is expressed by plasmocytoid DC

7,9

What cytokines do classical DC make

TNF, IL6, IL12, IL23

What cytokines do plasmacytoid DC make

Type 1 IFN

What is the function of classical DC

Induce T cell response

What is the function of plasmocytoid DC

Antiviral innate immunity and T cell induction

What domains are in MHC class I

alpha 1-3

What is the alpha chain associated with

beta2 microglobulin

What do alpha 1 and 2 domains do

Variation in floor of peptide binding groove

What does the alpha 3 domain do

Has a CD8T cell coreceptor binding site

What are the domains of MHC class II

alpha and beta

What do alpha1 and beta1 do

Form cleft

What do nonpolymorphic alpha2 and beta2 do

Have CD4 T cell coreceptor binding site

What to MHC bind to

peptide

How many peptides can MHC class I bind

8-9 amino acids

How many peptides can MHC class II bind to

10-30 amino acids

What are the properties of MHC peptide bonding

Each MHC displays one peptide at a time, broad specificity, surface expression of MHC requires bond peptide, very slow off rate

What are the genes of MHC class I

A B C

What are the genes of MHC class II

DP, DQ and DR

What are the properties of MHC

Co dominant and polymorphic

Where are MHC class I found

All nucleased cells (leukocyte, epithelial cell, mesenchymal cell)

What can recognize MHC class I

CD8 T cell

Where are MHC class II found

Dendritic cell, macrophage, B cell

What can recognize MHC class II

CD4 helper T

MHC class I recognize peptide from where

Cytosol

MHC class II recognize peptide from where

Endosome

DC function (as an APC)

Naive T activation

What is the effect of naive T activation

Clonal expansion and differentiation into effector T cell

Macrophage function (as APC)

Effector T cell activation

What is the effect of effector T cell activation for macrophage

Activation of macrophage

B cell function (as APC)

Effector T cell activation

What is the effect of Effector T cell activation (for B cell)

B cell activation and antibody production