rtpcr qpcr rna seq

RT-PCR Principle

Converts RNA to cDNA, then PCR-amplifies target genes.

RT-PCR Advantages

Low cost, simple.

RT-PCR Applications

Small-scale gene validation.

qPCR Principle

Quantifies cDNA in real-time using fluorescent probes (TaqMan/SYBR Green).

qPCR Advantages

High sensitivity, absolute quantification.

qPCR Applications

Clinical diagnostics (e.g., viral load).

RNA-seq Principle

Sequences all RNA fragments in a sample

RNA-seq Advantages

Genome-wide novel transcripts/splice variants.

RNA-seq Applications

Transcriptome cancer research