Lab Quiz 10 & 11

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16 Terms

1
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sheep red blood cells

to identify the colonial appearance of staphylococci through pigment and hemolytic activity

2
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7.5% salt

added in mannitol salt agar, creates a high concentration of salt/ hypertonic environment that inhibits gram-positive cocci

3
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mannitol

an indicator to differentiate S. aureus strains from S. epidermidis growing on it

4
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phenol red

a pH indicator, detects acid, will turn red/pink in high pH and yellow in low pH

5
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list 4 ways S. aureus can be distinguished from S. epidermidis

  • hemolysis

  • pigment and colony size

  • mannitol fermentation

  • salt tolerance

6
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how is the production of coagulase related to virulence

causes inappropriate clotting

7
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gram stain appearance of staphylococci

purple, grape like clusters

8
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why is it important to not remove any media when doing a catalase test from organisms growing on a blood agar plate?

blood will also fizz on it

9
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why is the catalase test used?

differentiate Streptococcus pyogenes and Staphylococcus aureus

10
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alpha hemolysis

incomplete breakdown of RBCs, oxidizes iron, makes green color

11
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beta hemolysis

complete destruction of RBCs, appears yellow/clear/transparent

12
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gamma hemolysis

no breakdown, no appearance change

13
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what type of hemolysis is demonstrated by normal throat flora?

alpha, beta, and gamma

14
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what type of hemolysis is displayed by streptococci that are most pathogenic for humans?

beta

15
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test that differentiates normal throat streptococci from Group A streptococci?

hemolysis and bacitracin sensitivity

16
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gram stain morphology of streptococci

gram-positive, cocci in chains