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SAR for beta 2 agonist activity
Amino substituent Needs to be larger than methyl. A bulky branched alkyl substituent is essential for beta activity [e.g., isopropyl (B1 =B2), t-butyl B2>B1]
Basic nitrogen atom (ionized (protonated) form interacts with receptor)
alpha-carbon substitution —> An ethyl substituent increases beta-selectivity by diminishing binding to the alpha-receptor. (Substituent larger than ethyl results in loss of binding from beta receptor.)
beta-OH group Essential for overall direct agonist activity; important for H-bonding (Needs to be in the R-configuration. Does not play a role in beta vs alpha selectivity)
Aromatic ring substituents: Catechol is essential but produces limited bioavailability due to chemical (orthoquinone formation) and metabolic instability (COMT), so Catechol modifications improve stability while still retaining activity.
Phenethanolamine Derivatives list
short acting beta 2 (SABAs), long acting beta 2 (LABAs), ultra long acting beta 2
long and ultra long acting SAR difference
big hydrophobic group attached to amine increases B2 receptor selectivity/affinity and DOA
B1 agonist SAR
Phenethylamine
as a catechol and a primary amine, dopamine is rapidly metabolized by COMT and MAO, has a short duration of action with no oral activity.
Some beta1 agonist activity
NO beta OH (decreases direct action)
ionizable amine