lecture 5 - stem cell identification/isolation

what are some common approaches to identifying stem cells?

• surface biomarkers

• metabolic activities

• cell cycle activities (or lack there of)

  • even the lack of cell cycle activities gives info about stemmness

are these identification approaches usually used in combination or individually?

used in combination

what are the two names that cell surface proteins can be known as to identify stem cells?

explain how the use of cell surface markers to identify stem cells works

A is necessary to identify this lineage but the surface markers change as you move down/cells differentiate so can be used to identify/isolate

explain how CD34 can be used in stem cell isolation/identification

how can monoclonal antibodies be used in stem cell identification?

describe strokes shift and how it can be used to identify stem cells

• measures excitability of a dye/signal

  

describe the benefits of confocal microscopy in studying stem cells

what is flow cytometry?

• provides information on the amount of protein present

  • can analyze 50K cells/min

• looks at both surface protein or internal proteins (if the cells are fixed)

what 3 features does flow cytometers work around?

how does flow cytometry work? what can it detect?

what are ABC transmembrane proteins and how can they be used to identify stem cells?

describe what is being expressed in this diagram

how can ALDH be used to identify stem cells?

how can Thymine be used to identify stem cells

how is quiescence helpful in stem cell identification?